...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Infection and immunity >Bacterial metabolism of human polymorphonuclear leukocyte-derived arachidonic acid.
【24h】

Bacterial metabolism of human polymorphonuclear leukocyte-derived arachidonic acid.

机译：人多形核白细胞衍生的花生四烯酸的细菌代谢。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Evidence for transcellular bacterial metabolism of phagocyte-derived arachidonic acid was sought by exposing human blood polymorphonuclear leukocytes, prelabelled with [3H]arachidonic acid, to opsonized, stationary-phase Pseudomonas aeruginosa (bacteria-to-phagocyte ratio of 50:1) for 90 min at 37 degrees C. Control leukocytes were stimulated with the calcium ionophore A23187 (5 microM) for 5 min. Radiochromatograms of arachidonic acid metabolites, extracted from A23187-stimulated cultures and then separated by reverse-phase high-performance liquid chromatography, revealed leukotriene B4, its omega-oxidation products, and 5-hydroxy-eicosatetraenoic acid. In contrast, two major metabolite peaks, distinct from known polymorphonuclear leukocyte arachidonic acid products by high-performance liquid chromatography or by thin-layer chromatography, were identified in cultures of P. aeruginosa with [3H]arachidonic acid-labelled polymorphonuclear leukocytes. Respective chromatographic characteristics of these novel products were identical to those of two major metabolite peaks produced by incubation of stationary-phase P. aeruginosa with [3H]arachidonic acid. Production of the metabolites was dependent upon pseudomonal viability. UV spectral data were consistent with a conjugated diene structure. Metabolism of arachidonic acid by P. aeruginosa was not influenced by the presence of catalase, superoxide dismutase, nordihydroguaiaretic acid, ethanol, dimethyl sulfoxide, or ferrous ions but was inhibited by carbon monoxide, ketoconazole, and 1,2-epoxy-3,3,3-trichloropropane. Our data suggest that pseudomonal metabolism of polymorphonuclear leukocyte-derived arachidonic acid occurs during phagocytosis, probably by enzymatic epoxidation and hydroxylation via an oxygenase. By this means, potential proinflammatory effects of arachidonic acid or its metabolites may be modulated by P. aeruginosa at sites of infection in vivo.
机译：通过将预先标记有[3H]花生四烯酸的人血多形核白细胞暴露于调理的固定相铜绿假单胞菌（细菌与吞噬细胞的比例为50：1）中，寻找吞噬细胞衍生的花生四烯酸的跨细胞细菌代谢证据在37℃下10分钟。用钙离子载体A23187（5microM）刺激对照白细胞5分钟。从A23187刺激的培养物中提取的花生四烯酸代谢物的放射色谱图，然后通过反相高效液相色谱进行分离，显示出白三烯B4，其ω-氧化产物和5-羟基二十碳四烯酸。相反，在铜绿假单胞菌的[3H]花生四烯酸标记多形核白细胞培养物中，鉴定出了两个主要代谢物峰，这两个峰是通过高效液相色谱法或薄层色谱法与已知的多形核白细胞花生四烯酸产物不同的。这些新产品的色谱特征与固定相铜绿假单胞菌与[3H]花生四烯酸温育产生的两个主要代谢物峰的色谱特征相同。代谢产物的产生取决于假单胞菌的生存能力。紫外光谱数据与共轭二烯结构一致。铜绿假单胞菌对花生四烯酸的代谢不受过氧化氢酶，超氧化物歧化酶，去甲二氢愈创木酸，乙醇，二甲基亚砜或亚铁离子的影响，但被一氧化碳，酮康唑和1,2-环氧-3,3抑制，3-三氯丙烷。我们的数据表明，多形核白细胞衍生的花生四烯酸在伪吞噬过程中会发生吞噬作用，这可能是由于酶的环氧化作用和经由加氧酶的羟化作用所致。通过这种方式，铜绿假单胞菌可在体内感染部位调节花生四烯酸或其代谢物的潜在促炎作用。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号