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首页> 外文期刊>Infection and immunity >Limited T-cell receptor beta-chain diversity of a T-helper cell type 1-like response to Mycobacterium leprae.
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Limited T-cell receptor beta-chain diversity of a T-helper cell type 1-like response to Mycobacterium leprae.

机译：对麻风分枝杆菌的T辅助细胞1型应答的有限T细胞受体β链多样性。

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Delayed-type hypersensitivity (DTH) is the standard measure of T-cell responsiveness to infectious organisms. For leprosy, the Mitsuda reaction, a local immune response to cutaneous challenge with Mycobacterium leprae, is considered to represent a measure of DTH against the pathogen. We analyzed the diversity of the T-cell receptor beta-chain repertoire in Mitsuda reactions to determine the breadth of the mycobacterial antigens involved. The polymerase chain reaction was used to compare V beta usage in the Mitsuda reaction T-cell lines established and unstimulated peripheral blood. These molecular analyses revealed a skewed T-cell receptor V beta gene usage in the Mitsuda reaction and in T-cell lines from lesions. To examine the reactivity of T cells from these lesions, T-cell lines were tested against the available native and recombinant antigens of M. leprae. T-cell lines derived from Mitsuda reactions responded more strongly to the 10-kDa M. leprae antigen, a homolog of GroES in Escherichia coli, than to other M. leprae proteins. T-cell lines were also shown to proliferate strongly in response to the 17- and 3-kDa proteins. The pattern of the lymphokine mRNA of these cells was reminiscent of the pattern of murine TH1 cells, positive for interleukin-2 and gamma interferon and weakly positive for interleukin-4. These data indicate that a limited array of T cells, perhaps recognizing stress proteins, secrete a type 1 lymphokine profile in the DTH response to mycobacteria.
机译：迟发型超敏反应（DTH）是T细胞对传染性生物反应的标准量度。对于麻风病，Mitsuda反应（一种针对麻风分枝杆菌的皮肤攻击的局部免疫反应）被视为代表针对病原体的DTH的量度。我们分析了Mitsuda反应中T细胞受体β链组成的多样性，以确定涉及的分枝杆菌抗原的广度。聚合酶链反应用于比较在建立的和未刺激的外周血的Mitsuda反应T细胞系中V beta的使用。这些分子分析揭示了在Mitsuda反应和来自病变的T细胞系中偏斜的T细胞受体Vβ基因的用法。为了检查来自这些损伤的T细胞的反应性，针对麻风分枝杆菌的可用天然和重组抗原测试了T细胞系。来自三田反应的T细胞系对10 kDa麻风分枝杆菌抗原（大肠杆菌中GroES的同源物）的反应比对其他麻风分枝杆菌蛋白的反应更强烈。 T细胞系还显示出对17kDa和3kDa蛋白的强烈反应。这些细胞的淋巴因子mRNA模式使人联想到鼠TH1细胞的模式，对白介素2和γ干扰素呈阳性，而对白介素4呈弱阳性。这些数据表明，可能识别应激蛋白的有限数量的T细胞在DTH对分枝杆菌的反应中分泌1型淋巴因子谱。

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