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首页> 外文期刊>Infection and immunity >Cloning and characterization of ECE1, a gene expressed in association with cell elongation of the dimorphic pathogen Candida albicans.
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Cloning and characterization of ECE1, a gene expressed in association with cell elongation of the dimorphic pathogen Candida albicans.

机译：ECE1的克隆和鉴定，该基因与双态病原体白色念珠菌的细胞伸长有关。

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摘要

The gene ECE1 (extent of cell elongation 1) was isolated by differential hybridization screening of a Candida albicans cDNA library by using probes derived from populations of yeast cells or hyphae. Expression of this gene was not detected when C. albicans grew as a budding yeast cell but was observed within 30 min after cells had been induced to form hyphae. In all strains tested, regardless of the induction signal, ECE1 expression correlated with the extent of cell elongation. The genomic version of ECE1 was cloned and sequenced. The deduced 271-amino-acid polypeptide consisted of eight tandem repeats of a degenerate 34-amino-acid sequence which contained no discernible homology with other known sequences. An ECE1 null mutant displayed no morphological alterations, which demonstrated that ECE1 is not essential for cell elongation or hypha formation despite the strict morphological association of its expression.
机译：通过使用来自酵母细胞或菌丝群体的探针对白色念珠菌cDNA文库进行差异杂交筛选，分离了基因ECE1（细胞延伸1的程度）。当白色念珠菌作为发芽的酵母细胞生长时未检测到该基因的表达，但在诱导细胞形成菌丝后30分钟内观察到了该基因。在所有测试的菌株中，无论诱导信号如何，ECE1表达均与细胞伸长程度相关。克隆并测序了ECE1的基因组版本。推导的271个氨基酸的多肽由简并的34个氨基酸的序列的八个串联重复组成，该序列不与其他已知序列具有可辨别的同源性。一个ECE1空突变体没有显示任何形态学改变，这表明ECE1尽管其表达具有严格的形态学联系，但对于细胞延长或菌丝形成不是必需的。

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