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首页> 外文期刊>Infection and immunity >Characterization of Candida albicans cell wall antigens with monoclonal antibodies.
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Characterization of Candida albicans cell wall antigens with monoclonal antibodies.

机译：用单克隆抗体表征白色念珠菌细胞壁抗原。

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The antigenic composition of Candida albicans is very complex. In order to study the antigenic relationship between blastoconidia and germ tubes of C. albicans, we produced several monoclonal antibodies and analyzed their reactivity against cell wall antigens either in intact cells or in cells treated with dithiothreitol. Overall, four types of reactivity were found. Monoclonal antibodies 3D9 and 15C9 stained the germ tubes only when tested by indirect immunofluorescence. However, they showed a different reactivity by immunoblotting. Monoclonal antibody 3D9 reacted with antigens with molecular masses of > 200 and 180 kDa specifically expressed in the germ tube. Monoclonal antibody 15C9 reacted with antigens of 87, 50, and 34 kDa present in the germ tube extract and with antigens of 92, 50, 34, and 32 kDa present in the blastoconidium extract. The reactivity of blastoconidia treated for different times with dithiothreitol with these monoclonal antibodies was also studied by enzyme-linked immunosorbent assay. The reactivity of monoclonal antibody 3D9 did not significantly change during the cell wall extraction. However, the reactivity of monoclonal antibody 15C9 was increased for blastoconidia extracted for 60 min and decreased markedly for blastocondia extracted for 120 min. Monoclonal antibody G3B was nonreactive by indirect immunofluoresence but reacted with antigens of 47 and 38 kDa present in the germ tube extract and with an antigen of 47 kDa present in the blastoconidium extract. Monoclonal antibody B9E stained both morphological phases by indirect immunofluorescence. By immunoblotting, it reacted with antigens of > 70 kDa present in the germ tube extract and with antigens of > 63, 56, 47, and 38 kDa present in the blastoconidium extract. Based on the results presented in this study, four types of antigens are described. Type I antigens are expressed on the outermost layers of the germ tube cell wall only. Type II antigens are expressed both on the germ tube cell wall surface and within the blastoconidium cell wall. Type III antigens are found within the cell wall of both blastoconidia and germ tubes. Type IV antigens are expressed on both the blastoconidium and germ tube surface. Two types more can be hypothesized for antigens expressed on the blastoconidium cell surface and within the germ tube cell wall (type V) and for those expressed on the blastoconidium surface only (type VI).
机译：白色念珠菌的抗原成分非常复杂。为了研究念珠菌和白色念珠菌胚管之间的抗原关系，我们生产了几种单克隆抗体，并分析了它们在完整细胞或在二硫苏糖醇处理的细胞中对细胞壁抗原的反应性。总体而言，发现了四种类型的反应性。仅当通过间接免疫荧光测试时，单克隆抗体3D9和15C9才将胚管染色。但是，它们通过免疫印迹显示出不同的反应性。单克隆抗体3D9与在生殖管中特异性表达的分子量> 200和180 kDa的抗原反应。单克隆抗体15C9与胚管提取物中存在的87、50和34 kDa抗原以及母囊胚提取物中存在的92、50、34和32 kDa抗原反应。还通过酶联免疫吸附试验研究了用二硫苏糖醇与这些单克隆抗体处理不同时间的酒球菌的反应性。单克隆抗体3D9的反应性在细胞壁提取过程中没有显着变化。但是，单克隆抗体15C9的反应性对于胚芽孢杆菌提取60分钟会增加，而对于胚芽孢杆菌提取120分钟则明显降低。单克隆抗体G3B通过间接免疫荧光不反应，但与胚管提取物中存在的47 kDa和38 kDa抗原以及母囊胚提取物中存在的47 kDa抗原反应。单克隆抗体B9E通过间接免疫荧光法对两个形态相染色。通过免疫印迹，它与种管提取物中存在的> 70 kDa的抗原以及在胚芽孢杆菌提取物中存在的> 63、56、47和38 kDa的抗原反应。基于这项研究中提出的结果，描述了四种类型的抗原。 I型抗原仅在生殖管细胞壁的最外层表达。 II型抗原在胚管细胞壁表面和母囊胚细胞壁内表达。 III型抗原被发现在母细胞和胚芽管的细胞壁内。 IV型抗原在母囊胚和胚管表面均表达。对于在胚芽孢囊细胞表面上和胚管细胞壁内表达的抗原（V型）和仅在胚芽孢囊表面上表达的抗原（VI型），可以假设两种类型。

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