• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Infection and immunity >Contact of lymphocytes with Helicobacter pylori augments natural killer cell activity and induces production of gamma interferon.
【24h】

Contact of lymphocytes with Helicobacter pylori augments natural killer cell activity and induces production of gamma interferon.

机译：淋巴细胞与幽门螺杆菌的接触增强了自然杀伤细胞的活性并诱导了γ干扰素的产生。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

We studied the capacity of glutaraldehyde-fixed Helicobacter pylori to stimulate natural killer (NK) cell activity. Bacteria were incubated overnight with peripheral blood lymphocytes enriched for large granular lymphocytes (LGL), the mediators of non-major histocompatibility complex-restricted cellular cytotoxicity. Then, the cytolytic activity of LGL was tested against various tumor target cells. We observed that efficient cytolytic activity was generated against resistant and nonresistant tumor target cell lines. Nine local clinical isolates of H. pylori and the reference strain NCTC 11637 were tested, and they all were equally effective in inducing NK cell activity. However, flagellin antigen, glycine extract, urease, and lipopolysaccharide prepared from H. pylori NCTC 11637 all failed to induce significant NK cell activity. The supernatants which were collected after coincubation of bacteria with LGL contained a factor(s) which could activate resting LGL into efficient cytolytic activity. The supernatants were also analyzed for interferon (IFN) activity. We observed that high titers of IFN were produced and that IFN activity was neutralized with anti-gamma interferon (IFN-gamma) antiserum, but not with anti-IFN-alpha antiserum. Thus, contact of lymphocytes with H. pylori leads to efficient stimulation of NK cell activity and the production of IFN-gamma.
机译：我们研究了戊二醛固定的幽门螺杆菌刺激自然杀伤（NK）细胞活性的能力。细菌与富含大颗粒淋巴细胞（LGL）的外周血淋巴细胞孵育过夜，大颗粒淋巴细胞是非主要组织相容性复合物限制细胞毒性的介质。然后，测试了LGL对各种肿瘤靶细胞的溶细胞活性。我们观察到针对抗性和非抗性肿瘤靶细胞系产生了有效的溶细胞活性。测试了9例幽门螺杆菌的局部临床分离株和参考菌株NCTC 11637，它们在诱导NK细胞活性方面均同样有效。然而，由幽门螺杆菌NCTC 11637制备的鞭毛蛋白抗原，甘氨酸提取物，脲酶和脂多糖均不能诱导明显的NK细胞活性。将细菌与LGL共孵育后收集的上清液含有一种或多种因子，可以将静止的LG​​L激活为有效的细胞溶解活性。还分析上清液的干扰素（IFN）活性。我们观察到产生了高滴度的IFN，并且抗γ-干扰素（IFN-γ）抗血清中和了IFN活性，而抗IFN-α抗血清却没有。因此，淋巴细胞与幽门螺杆菌的接触导致NK细胞活性的有效刺激和IFN-γ的产生。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号