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首页> 外文期刊>Infection and immunity >Immunological characteristics of a synthetic peptide associated with a catalytic domain of mutans streptococcal glucosyltransferase.
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Immunological characteristics of a synthetic peptide associated with a catalytic domain of mutans streptococcal glucosyltransferase.

机译：与变形链球菌葡糖基转移酶的催化结构域相关的合成肽的免疫学特性。

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The immunogenicity of a multiple antigenic peptide construct consisting of four copies of the synthetic 21-mer peptide DANFDSIRVDAVDNVDADLLQ was measured. The composition of this peptide was derived from a sequence in the N-terminal region of mutans streptococcal glucosyltransferases (GTFs) containing an aspartic acid implicated in catalysis. The peptide (CAT) construct was synthesized as a tetramer on a lysine backbone and subcutaneously injected into Sprague-Dawley rats for polyclonal antibody formation or intraperitoneally injected into BALB/c mice, and then spleen cell fused with Sp2/0Ag14 murine myeloma cells for monoclonal antibody formation. The resulting rat antisera and mouse monoclonal antibodies reacted with CAT and with native GTF isozymes from Streptococcus sobrinus and Streptococcus mutans (in enzyme-linked immunosorbent assay and Western blot [immunoblot] analyses). Functional inhibition of the water-insoluble glucan synthetic activity of S. sobrinus GTF-I was demonstrated with an immunoglobulin M anti-CAT monoclonal antibody (> 80% inhibited) and with rat sera (approximately 17% inhibited). The monoclonal antibody preparation also modestly inhibited the water-soluble glucan synthetic activity of an S. mutans GTF mixture. These results suggest that the CAT peptide contains B-cell epitopes that are similar to those of intact mutans streptococcal GTFs and has the potential to elicit antibody that can inhibit GTF function. Thus, sequences within this peptide construct may have value for inclusion in a synthetic dental caries vaccine.
机译：测量了由四个拷贝的合成21聚体肽DANFDSIRVDAVDNVDADLLQ组成的多抗原肽构建体的免疫原性。该肽的组成衍生自变形链球菌葡糖基转移酶（GTF）N端区域的序列，该序列包含催化作用的天冬氨酸。将肽（CAT）构建体合成为赖氨酸骨架上的四聚体，并皮下注射到Sprague-Dawley大鼠中以形成多克隆抗体，或腹膜内注射到BALB / c小鼠中，然后将脾细胞与Sp2 / 0Ag14鼠骨髓瘤细胞融合在一起作为单克隆抗体抗体形成。所得的大鼠抗血清和小鼠单克隆抗体与CAT以及来自链球菌和变形链球菌的天然GTF同工酶反应（在酶联免疫吸附试验和Western blot [免疫印迹]分析中）。用免疫球蛋白M抗CAT单克隆抗体（抑制了＞ 80％）和大鼠血清（抑制了约17％）证明了对链球菌GTF-1的水不溶性葡聚糖合成活性的功能抑制。单克隆抗体制剂还适度抑制变形链球菌GTF混合物的水溶性葡聚糖合成活性。这些结果表明，CAT肽包含与完整变异链球菌GTF相似的B细胞表位，并具有引发可抑制GTF功能的抗体的潜力。因此，该肽构建体中的序列可能具有包含在合成龋齿疫苗中的价值。

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