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首页> 外文期刊>Infection and immunity >Protein Hpn: cloning and characterization of a histidine-rich metal-binding polypeptide in Helicobacter pylori and Helicobacter mustelae.
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Protein Hpn: cloning and characterization of a histidine-rich metal-binding polypeptide in Helicobacter pylori and Helicobacter mustelae.

机译：Hpn蛋白：幽门螺杆菌和芥子油杆菌中富含组氨酸的金属结合多肽的克隆和鉴定。

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Helicobacter pylori is a human gastrointestinal pathogen involved in gastritis, duodenal ulcers, and gastric neoplasia. This microorganism produces large amounts of a urease which, like all known ureases, has nickel in the active site. We have identified a protein in clinical isolates of H. pylori and an identical protein in the ferret pathogen Helicobacter mustelae that strongly binds Ni2+ and Zn2+. This protein has been named Hpn to emphasize its origins in H. pylori and its affinity for nickel. The encoding hpn gene, cloned and expressed in Escherichia coli ER1793, has an open reading frame (180 bp) that specifies a protein with a calculated molecular mass of 7,077 Da and with the same amino-terminal sequence as that of wild-type Hpn. The deduced sequence of Hpn consists of 60 amino acids, of which 28 (47%) are histidines. The hpn gene does not map with the urease gene cluster on the H. pylori chromosome. An Hpn-negative, isogenic H. pylori strain, generated by hpn gene deletion and grown on blood agar, had the same urease activity that wild-type cells did. Thus, the role of Hpn in helicobacters is unknown.
机译：幽门螺杆菌是一种人类胃肠道病原体，涉及胃炎，十二指肠溃疡和胃肿瘤。这种微生物产生大量的脲酶，就像所有已知的脲酶一样，在活性位点具有镍。我们已经在幽门螺杆菌的临床分离物中鉴定出一种蛋白质，并且在雪貂病原体幽门螺杆菌中发现了一种与Ni2 +和Zn2 +紧密结合的相同蛋白质。该蛋白已被命名为Hpn，以强调其起源于幽门螺杆菌及其对镍的亲和力。克隆并在大肠杆菌ER1793中表达的编码hpn基因具有开放阅读框（180 bp），该框架指定了一种蛋白质，其计算分子量为7,077 Da，并且其氨基末端序列与野生型Hpn相同。 Hpn的推导序列由60个氨基酸组成，其中28个（47％）为组氨酸。 hpn基因未与幽门螺杆菌染色体上的脲酶基因簇作图。由hpn基因缺失产生并在血琼脂上生长的Hpn阴性同基因幽门螺杆菌菌株具有与野生型细胞相同的脲酶活性。因此，Hpn在幽门螺杆菌中的作用尚不清楚。

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