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首页> 外文期刊>Infection and immunity >Helicobacter pylori cytotoxin: importance of native conformation for induction of neutralizing antibodies.
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Helicobacter pylori cytotoxin: importance of native conformation for induction of neutralizing antibodies.

机译：幽门螺杆菌细胞毒素：天然构象对于诱导中和抗体的重要性。

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We have attempted to express the Helicobacter pylori vacuolating cytotoxin in Escherichia coli. Although the 95-kDa VacA polypeptide was expressed abundantly, it completely lacked any biological activity. In addition, this material failed to induce neutralizing antibodies after immunization of rabbits. In contrast, highly purified high-molecular-mass cytotoxin from the supernatant of H. pylori cultures was active in a HeLa cell assay and effectively induced a neutralizing response in rabbits. Neutralizing sera were shown to contain a high proportion of antibodies which recognized conformational epitopes found only on the native toxin. The data indicate that toxin-neutralizing epitopes are conformational and that potential vaccines based on the cytotoxin may benefit from the use of the intact molecule.
机译：我们试图在大肠杆菌中表达幽门螺杆菌空泡化细胞毒素。尽管95-kDa VacA多肽被大量表达，但是它完全缺乏任何生物学活性。另外，这种材料在兔免疫后不能诱导中和抗体。相反，来自幽门螺杆菌培养物上清液的高度纯化的高分子细胞毒素在HeLa细胞测定中具有活性，并在兔中有效诱导中和反应。显示中和血清包含高比例的抗体，这些抗体识别仅在天然毒素上发现的构象表位。数据表明，毒素中和表位是构象的，基于细胞毒素的潜在疫苗可能会受益于完整分子的使用。

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