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首页> 外文期刊>Infection and immunity >Variation in antigenicity and infectivity of derivatives of Borrelia burgdorferi, strain B31, maintained in the natural, zoonotic cycle compared with maintenance in culture.
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Variation in antigenicity and infectivity of derivatives of Borrelia burgdorferi, strain B31, maintained in the natural, zoonotic cycle compared with maintenance in culture.

机译：与在培养中维持相比，在自然，人畜共患的周期中维持的伯氏疏螺旋体B31菌株的衍生物的抗原性和感染性的变化。

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The original isolate of Borrelia burgdorferi, strain B31, can be maintained in vitro indefinitely. A number of studies have demonstrated that there are recognizable changes in the genetic composition of the spirochete after more than 60 passages. We have maintained B31 in the natural zoonotic cycle of transmission of infection between laboratory mice and laboratory-reared Ixodes ticks. To determine whether similar changes occur in the natural transmission cycle, we reisolated strain B31 from mouse skin at the fifth zoonotic cycle. This reisolated derivative had the same infectivity as the parent B31 strain, had lost the 8-kb supercoiled plasmid present in B31, and induced a gross serum antibody response indistinguishable from the B31 immune response. Analysis of antigen expression with monoclonal antibodies generated against B31, however, showed differential expression of a subset of antigens between B31 and the isolated derivative.
机译：伯氏疏螺旋体的原始分离株B31可以在体外无限期保存。多项研究表明，经过60多个传代后，螺旋体的遗传组成发生了可识别的变化。我们一直将B31维持在实验室小鼠和实验室饲养的速激肽之间的自然传播过程中。为了确定在自然传播周期中是否发生类似的变化，我们在第五个人畜共患病周期中从小鼠皮肤中重新分离了菌株B31。该重新分离的衍生物具有与亲本B31菌株相同的感染性，已经丢失了存在于B31中的8kb超螺旋质粒，并且诱导了与B31免疫应答无法区分的总血清抗体应答。然而，用针对B31的单克隆抗体对抗原表达进行的分析显示，B31与分离的衍生物之间的抗原亚群表达差异。

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