Identification of shuA, the gene encoding the heme receptor of Shigella dysenteriae, and analysis of invasion and intracellular multiplication of a shuA mutant.

M Mills; S M Payne

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Identification of shuA, the gene encoding the heme receptor of Shigella dysenteriae, and analysis of invasion and intracellular multiplication of a shuA mutant.

机译：鉴定shuA，编码痢疾志贺氏菌血红素受体的基因shuA，并分析shuA突变体的侵袭和细胞内增殖。

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shuA encodes a 70-kDa outer membrane heme receptor in Shigella dysenteriae. Analysis of the shuA DNA sequence indicates that this gene encodes a protein with homology to TonB-dependent receptors of gram-negative bacteria. Transport of heme by the ShuA protein requires TonB and its accessory proteins ExbB and ExbD. The shuA DNA sequence contains a putative Fur box overlapping the -10 region of a potential shuA promoter, and the expression of shuA is repressed by exogenous iron or hemin in a Fur-dependent manner, although hemin repressed expression to a lesser extent than iron salts. Disruption of this open reading frame on the S. dysenteriae chromosome by marker exchange yielded a strain that failed to use heme as an iron source, indicating that shuA is essential for heme transport in S. dysenteriae. However, shuA is not essential for invasion or multiplication within cultured Henle cells; the shuA mutant invaded and produced normal plaques in confluent cell monolayers.

机译：shuA在痢疾志贺氏菌中编码一个70 kDa的外膜血红素受体。 shuA DNA序列的分析表明，该基因编码的蛋白质与革兰氏阴性细菌的TonB依赖受体具有同源性。 ShuA蛋白转运血红素需要TonB及其辅助蛋白ExbB和ExbD。 shuA DNA序列包含一个与潜在shuA启动子-10区重叠的假定的Fur盒，并且shuA的表达被外源铁或血红素以Fur依赖的方式抑制，尽管hemin抑制的表达程度小于铁盐。通过标志物交换破坏痢疾链球菌染色体上的这个开放阅读框，产生了不能使用血红素作为铁源的菌株，表明shuA对于痢疾链球菌中血红素的运输至关重要。但是，shuA对于培养的Henle细胞内的侵袭或增殖不是必需的; shuA突变体在融合细胞单层中侵入并产生正常噬菌斑。

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《Infection and immunity》 |1997年第12期|共6页
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M Mills; S M Payne;
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6. Identification of shuA the gene encoding the heme receptor of Shigella dysenteriae and analysis of invasion and intracellular multiplication of a shuA mutant. [O] . M Mills, S M Payne 1997

机译：鉴定shuA编码痢疾志贺氏菌血红素受体的基因并分析shuA突变体的侵袭和细胞内增殖。
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Identification of shuA, the gene encoding the heme receptor of Shigella dysenteriae, and analysis of invasion and intracellular multiplication of a shuA mutant.

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