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首页> 外文期刊>Infection and immunity >Expression of Two Members of the pMGA Gene Family of Mycoplasma gallisepticum Oscillates and Is Influenced by pMGA-Specific Antibodies
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Expression of Two Members of the pMGA Gene Family of Mycoplasma gallisepticum Oscillates and Is Influenced by pMGA-Specific Antibodies

机译：鸡毒支原体pMGA基因家族的两个成员的表达振荡，并受pMGA特异性抗体的影响。

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摘要

Certain monoclonal antibodies and polyclonal antisera directed to pMGA, the major protein of Mycoplasma gallisepticum, were tested for the ability to influence the surface phenotype of the cell population which resulted from their inclusion in growth medium. The polyclonal antiserum and one monoclonal antibody (MAb 66) resulted in an alteration of surface phenotype; specifically, populations of cells grown either on plates or in broth cultures which contained these reagents ceased the expression of pMGA and instead expressed an antigenically unrelated new polypeptide (p82). Upon the removal of antibody, the progeny of these cells regained pMGA expression and produced antigenically sectored colonies. The basis of this switch between pMGA+ and pMGA? states was shown to be transcriptional. The p82 polypeptide, the expression of which resulted from growth of cells in antibodies, was another member of the pMGA gene family and was located just downstream from the pMGA gene normally expressed by the M. gallisepticum cells used. Collectively the results of this work suggest that this organism has evolved an unusual means of altering the antigenic composition of its surface in response to antibodies or to other environmental cues.
机译：测试了某些针对pMGA（鸡支原体支原体）的主要蛋白的单克隆抗体和多克隆抗血清的影响细胞群表面表型的能力，这些能力是由于它们被包含在生长培养基中而引起的。多克隆抗血清和一种单克隆抗体（MAb 66）导致表面表型的改变;具体而言，在含有这些试剂的平板或肉汤培养物中生长的细胞群停止了pMGA的表达，而是表达了与抗原无关的新多肽（p82）。去除抗体后，这些细胞的后代恢复了pMGA表达并产生了抗原扇形集落。 pMGA + 和pMGA ？状态之间这种转换的基础被证明是转录的。 p82多肽的表达是由抗体中的细胞生长引起的，是pMGA基因家族的另一个成员，位于通常由 M表达的pMGA基因的下游。使用的鸡败血性细胞。总的来说，这项工作的结果表明，这种生物体已经进化出一种不寻常的方法，可以响应抗体或其他环境因素而改变其表面的抗原成分。

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