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首页> 外文期刊>Infection and immunity >Avidity as a Determinant of the Protective Efficacy of Human Antibodies to Pneumococcal Capsular Polysaccharides
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Avidity as a Determinant of the Protective Efficacy of Human Antibodies to Pneumococcal Capsular Polysaccharides

机译：亲和力作为人源抗体对肺炎球菌荚膜多糖保护作用的决定因素

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Antibodies reactive with capsular polysaccharides are considered the principal mediators of immunity against invasive diseases caused byStreptococcus pneumoniae. In this study, we tested the hypothesis that anti-pneumococcal capsular polysaccharide (PPS) antibody avidity can influence protective efficacy. We measured the avidities of individual adult postvaccination immunoglobulin G2 (IgG2) antibodies to PPS serotypes 6B and 23F and examined the relationship between avidity and opsonophagocytic and mouse-protective activities. The avidities of PPS 6B- and PPS 23F-specific IgG2 antibodies ranged from 6 to 31 nM?1 and from 3 to 20 nM?1, respectively. We observed an inverse correlation between the magnitude of avidity and the amount of antibody required to protect mice against lethal bacteremia caused by serotype 6B pneumococci. Similarly, higher-avidity antibodies were more effective than lower-avidity antibodies in vitro in mediating complement-dependent opsonophagocytosis of both 6B and 23F pneumococci. These data suggest that in adults, PPS antibodies are sufficiently polymorphic to possess biologically significant variations in avidity. We conclude that avidity functions as an important determinant of anticapsular antibody protective efficacy against pneumococci.
机译：与荚膜多糖反应的抗体被认为是抗肺炎链球菌引起的侵袭性疾病免疫力的主要介质。在这项研究中，我们测试了抗肺炎球菌荚膜多糖（PPS）抗体亲和力会影响保护功效的假设。我们测量了成人成年疫苗接种后免疫球蛋白G2（IgG2）抗体对PPS血清型6B和23F的亲和力，并检验了亲和力与调理吞噬细胞和小鼠保护活性之间的关系。 PPS 6B和PPS 23F特异性IgG2抗体的亲和力分别为6至31 nM ？1 和3至20 nM ？1 。我们观察到亲和力的大小与保护小鼠免受由血清型6B肺炎球菌引起的致死菌血症所需的抗体量成反比。类似地，在介导6B和23F肺炎球菌的补体依赖性调理吞噬作用中，较高亲和力的抗体在体外比较低亲和力的抗体更有效。这些数据表明，在成年人中，PPS抗体具有足够的多态性，可以在生物学上具有明显的亲和力变异。我们得出结论，亲和力是抗肺炎球菌的抗荚膜抗体保护功效的重要决定因素。

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