• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Infection and immunity >T-Cell Epitope Mapping of the Three Most Abundant Extracellular Proteins of Mycobacterium tuberculosis in Outbred Guinea Pigs
【24h】

T-Cell Epitope Mapping of the Three Most Abundant Extracellular Proteins of Mycobacterium tuberculosis in Outbred Guinea Pigs

机译：近亲豚鼠结核分枝杆菌中三种最丰富的细胞外蛋白的T细胞表位定位

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The three most abundant extracellular proteins ofMycobacterium tuberculosis, the 30-, 32-, and 16-kDa major extracellular proteins, are particularly promising vaccine candidates. We have mapped T-cell epitopes of these three proteins in outbred guinea pigs by immunizing the animals with each protein and assaying splenic lymphocyte proliferation against a series of overlapping synthetic peptides covering the entire length of the mature proteins. The 30-kDa protein contained nine immunodominant epitopes, the 32-kDa protein contained two immunodominant epitopes, and the 16-kDa protein contained a highly immunodominant region at its N terminus. The immunodominant epitopes of the 30- and 32-kDa proteins in outbred guinea pigs were frequently identified in healthy purified-protein-derivative-positive or BCG-vaccinated individuals in previous studies. The immunodominant epitopes of these major extracellular proteins have potential utility in an epitope-based vaccine against tuberculosis.
机译：结核分枝杆菌的三种最丰富的细胞外蛋白，即30、32和16 kDa的主要细胞外蛋白，是特别有希望的疫苗候选物。我们通过将每种蛋白质免疫动物并针对覆盖成熟蛋白质全长的一系列重叠合成肽测定脾淋巴细胞增殖，从而绘制了近交豚鼠这三种蛋白质的T细胞表位。 30 kDa蛋白质包含9个免疫显性表位，32 kDa蛋白质包含2个免疫显性表位，而16 kDa蛋白质在其N端包含一个高度免疫显性的区域。在以前的研究中，经常在健康的纯化蛋白衍生阳性或BCG疫苗接种的个体中鉴定出近交豚鼠中30 kDa和32 kDa蛋白的免疫显性表位。这些主要细胞外蛋白的免疫显性表位在基于表位的抗结核疫苗中具有潜在的实用性。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号