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首页> 外文期刊>Infection and immunity >Campylobacter jejuni-Stimulated Secretion of Interleukin-8 by INT407 Cells
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Campylobacter jejuni-Stimulated Secretion of Interleukin-8 by INT407 Cells

机译：空肠弯曲杆菌刺激INT407细胞分泌白介素8

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摘要

Incubation of INT407 cells with various clinical isolates ofCampylobacter jejuni resulted in secretion of interleukin-8 (IL-8) at levels ranging from 96 to 554 pg/ml at 24 h. The strains which produced the highest levels of IL-8 secretion were 81-176 and BT44. Induction of IL-8 secretion required live cells of 81-176 and was dependent on de novo protein synthesis. Site-specific mutants of 81-176, which were previously shown to be defective in adherence and invasion, resulted in reduced levels of secretion of IL-8, andcheY mutants of strains 81-176 and 749, which are hyperadherent and hyperinvasive, resulted in higher levels of IL-8 secretion. Another mutant of 81-176, which adheres at about 43% of the wild-type levels but is noninvasive, also showed marked reduction in IL-8 levels, suggesting that invasion is necessary for high levels of IL-8 secretion. When gentamicin was added to INT407 cells at 2 h after infection with 81-176, IL-8 secretion 22 h later was equivalent to that of controls without gentamicin, suggesting that the events which trigger induction and release of IL-8 occur early in the interactions of bacteria and eukaryotic cells.
机译：将INT407细胞与空肠弯曲杆菌的各种临床分离株一起孵育后，在24小时内分泌白介素8（IL-8）的水平范围为96至554 pg / ml。产生最高水平的IL-8分泌的菌株是81-176和BT44。 IL-8分泌的诱导需要81-176的活细胞，并依赖于从头合成蛋白质。先前显示在黏附和侵袭方面存在缺陷的81-176位点特异性突变体导致IL-8分泌水平降低，而81-176和749株的 cheY 突变体，它们是高粘附性和高侵袭性的，导致较高水平的IL-8分泌。 81-176的另一种突变体，其粘附程度约为野生型水平的43％，但是非侵入性的，也显示出IL-8水平显着降低，表明入侵是高水平IL-8分泌所必需的。当在81-176感染后2小时将庆大霉素添加到INT407细胞中时，22小时后IL-8的分泌与不含庆大霉素的对照组相同，这提示触发IL-8诱导和释放的事件发生在早期。细菌与真核细胞的相互作用。

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