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首页> 外文期刊>Infection and immunity >Isogenic Lysogens of Diverse Shiga Toxin 2-Encoding Bacteriophages Produce Markedly Different Amounts of Shiga Toxin
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Isogenic Lysogens of Diverse Shiga Toxin 2-Encoding Bacteriophages Produce Markedly Different Amounts of Shiga Toxin

机译：多种志贺毒素2-编码噬菌体的等基因溶菌原产生明显不同量的志贺毒素

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摘要

We produced isogenic Escherichia coli K-12 lysogens of seven different Shiga toxin 2 (Stx2)-encoding bacteriophages derived from clinical Shiga toxin-producing E. coli (STEC) isolates of serotypes O157:H7, O145, O111, and O83 to assess the variability among these phages and determine if there were phage-related differences in toxin production. Phage genomic restriction fragment length polymorphisms (RFLP) and superinfection resistance studies revealed significant differences among these phages and allowed the seven phages to be placed into five distinct groups. Experiments revealed striking differences in spontaneous phage and toxin production that were correlated with the groupings derived from the RFLP and resistance studies. These results suggest that the genotype of the Stx2 prophage can influence the level of phage release and toxin expression by host strains and thus may be relevant to STEC pathogenesis.
机译：我们生产了7种不同的志贺毒素K2溶原菌，它们来自临床产生志贺毒素的 E，其中有7种不同的志贺毒素2（Stx2）编码噬菌体。大肠杆菌（STEC）分离株的血清型为O157：H7，O145，O111和O83，以评估这些噬菌体之间的变异性，并确定毒素生产中是否存在与噬菌体相关的差异。噬菌体基因组限制性片段长度多态性（RFLP）和抗超级感染研究表明，这些噬菌体之间存在显着差异，并将这七个噬菌体分为五个不同的组。实验表明，自发噬菌体和毒素产生的显着差异与RFLP和耐药性研究得出的分组有关。这些结果表明Stx2噬菌体的基因型可以影响宿主菌株的噬菌体释放水平和毒素表达水平，因此可能与STEC发病机制有关。

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