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首页> 外文期刊>Infection and immunity >Homotypic and Heterotypic Antibody Responses to a 56-Kilodalton Protein of Orientia tsutsugamushi
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Homotypic and Heterotypic Antibody Responses to a 56-Kilodalton Protein of Orientia tsutsugamushi

机译：同种和异型抗体对Or虫东方虫的56千达尔顿蛋白质的反应。

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We analyzed homotypic and heterotypic antibody responses to a type-specific antigen (Tsa), a 56-kDa protein of Orientia tsutsugamushi, by using sera from mice immunized with strains Gilliam, Karp, Kato, and Boryong. We generated a series of deletion constructs of the tsa gene and expressed them as MalE fusion proteins. Variable domain I (VD I) showed strong responses to homotypic antibodies. Antigenic domain II (AD II) from Boryong and Karp showed cross-reactivities to each other. VD III showed no responses to any of the antibodies. Sera from Kato-immunized mice showed only homotypic responses to AD III. On the other hand, sera of the mice immunized with Gilliam, Karp, or Boryong showed homotypic as well as heterotypic responses to this region. VD IV showed the strongest heterotypic antibody responses among the fragments tested. These data suggest that VD I is important in homotypic antibody responses and that AD II, AD III, and VD IV are important in heterotypic antibody responses of the mice to Tsa.
机译：我们通过使用Gilliam，Karp，Kato和Boryong菌株免疫小鼠的血清，分析了对一种类型特异性抗原（Tsa）的同源型和异型抗体反应，Tsa是tsu虫东方Or的56 kDa蛋白。我们生成了 tsa 基因的一系列缺失构建体，并将其表达为MalE融合蛋白。可变域I（VD I）对同型抗体表现出强烈的反应。来自Boryong和Karp的抗原结构域II（AD II）显示出彼此的交叉反应性。 VD III没有显示对任何抗体的反应。来自加藤免疫的小鼠的血清仅显示出对AD III的同型应答。另一方面，用Gilliam，Karp或Boryong免疫的小鼠血清对该区域表现出同型和异型反应。 VD IV在测试的片段中显示出最强的异型抗体反应。这些数据表明，VD I在同型抗体应答中很重要，而AD II，AD III和VD IV在小鼠对Tsa的异型抗体应答中很重要。

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