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首页> 外文期刊>Infection and immunity >Intracellular Trafficking of Brucella abortus in J774 Macrophages
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Intracellular Trafficking of Brucella abortus in J774 Macrophages

机译：J774巨噬细胞中布鲁氏菌流产的细胞内贩运

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Brucella abortus is a facultative intracellular bacterium capable of surviving inside professional and nonprofessional phagocytes. The microorganism remains in membrane-bound compartments that in several cell types resemble modified endoplasmic reticulum structures. To monitor the intracellular transport of B. abortus in macrophages, the kinetics of fusion of phagosomes with preformed lysosomes labeled with colloidal gold particles was observed by electron microscopy. The results indicated that phagosomes containing live B. abortus were reluctant to fuse with lysosomes. Furthermore, newly endocytosed material was not incorporated into these phagosomes. These observations indicate that the bacteria strongly affect the normal maturation process of macrophage phagosomes. However, after overnight incubation, a significant percentage of the microorganisms were found in large phagosomes containing gold particles, resembling phagolysosomes. Most of theBrucella bacteria present in phagolysosomes were not morphologically altered, suggesting that they can also resist the harsh conditions prevalent in this compartment. About 50% colocalization ofB. abortus with LysoSensor, a weak base that accumulates in acidic compartments, was observed, indicating that theB. abortus bacteria do not prevent phagosome acidification. In contrast to what has been described for HeLa cells, only a minor percentage of the microorganisms were found in compartments labeled with monodansylcadaverine, a marker for autophagosomes, and with DiOC6 (3,3′-dihexyloxacarbocyanine iodide), a marker for the endoplasmic reticulum. These results indicate thatB. abortus bacteria alter phagosome maturation in macrophages. However, acidification does occur in these phagosomes, and some of them can eventually mature to phagolysosomes.
机译：流产布鲁氏菌是一种兼性的细胞内细菌，能够在专业和非专业吞噬细胞内存活。微生物保留在膜结合的区室中，该区室在几种细胞类型中类似于修饰的内质网结构。为了监测巨噬细胞中流产布鲁氏菌的细胞内运输，通过电子显微镜观察了吞噬体与预先形成的用胶体金颗粒标记的溶酶体融合的动力学。结果表明，含有活流产双歧杆菌的吞噬体不愿与溶酶体融合。此外，新的内吞材料未掺入这些吞噬体中。这些观察结果表明细菌强烈影响巨噬细胞吞噬体的正常成熟过程。然而，在过夜孵育后，在包含金颗粒的大吞噬体中发现了很大百分比的微生物，类似于吞噬体。吞噬溶酶体中存在的大多数布氏杆菌细菌在形态上没有改变，表明它们还可以抵抗在该区室中普遍存在的恶劣条件。 B约50％的共定位。观察到用LysoSensor（在酸性区室中积累的弱碱）流产，表明B。流产细菌不能阻止吞噬体酸化。与针对HeLa细胞的描述相反，在隔室中仅发现了少量微生物，这些隔室被标记为单丹磺酰尸胺（自噬小体的标记）和DiOC6（3,3'-二己基氧杂碳菁碘化物）作为内质网的标记。这些结果表明B。流产细菌改变巨噬细胞的吞噬体成熟。但是，在这些吞噬体中确实会发生酸化，并且其中一些最终可以成熟为吞噬体。

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