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首页> 外文期刊>Infection and immunity >Eimeria tenella Infection Induces Local Gamma Interferon Production and Intestinal Lymphocyte Subpopulation Changes
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Eimeria tenella Infection Induces Local Gamma Interferon Production and Intestinal Lymphocyte Subpopulation Changes

机译：Eimeria tenella感染诱导局部γ干扰素的产生和肠道淋巴细胞亚群的变化。

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The role of intestinal lymphocytes and gamma interferon (IFN-γ) production in protective immunity to Eimeria tenellainfection was evaluated in two inbred strains of chickens (SC and TK) that display different patterns of susceptibility to coccidiosis. Oral inoculation of either strain with E. tenella led to parasite invasion of the intestinal cecum and cecal tonsils. Greater fecal oocyst shedding was seen in TK chickens. Flow cytometric analyses of cecal tonsil lymphocytes demonstrated greater numbers of CD4+ and T-cell receptor γδ-positive (TCR1+) cells in SC chickens and elevated numbers of CD8+ and TCR2+ cells in TK chickens following primary infection. IFN-γ mRNA expression was significantly increased in cecal tonsil and intraepithelial lymphocytes at days 6 and 8, respectively, after primary infection in SC compared to TK chickens. While no differences were noted between cecal tonsil lymphocytes of the two strains following secondary infection, TK chickens showed elevated IFN-γ transcript levels in intestinal intraepithelial lymphocytes at this time. Selective depletion of CD4+, but not CD8+, cecal tonsil lymphocytes in SC chickens resulted in a reduced IFN-γ mRNA expression, indicating that CD4+ cells are the primary source of this cytokine. Collectively, these results indicate that local lymphocyte responses and production of IFN-γ are influenced by host genetic factors.
机译：在表现出对球虫病易感性不同的两种自交系鸡（SC和TK）中，评估了肠道淋巴细胞和γ-干扰素（IFN-γ）的产生在保护性对艾美球虫的保护性免疫中的作用。任一种菌株的口服接种都将导致大肠杆菌盲肠和盲肠扁桃体的寄生虫入侵。 TK鸡见到更大的粪便卵囊脱落。盲肠扁桃体淋巴细胞的流式细胞仪分析表明，SC鸡中CD4 +和T细胞受体γδ阳性（TCR1 +）细胞数量增多，而TK鸡中CD8 +和TCR2 +细胞数量增加。与TK鸡相比，SC初次感染后，盲肠扁桃体和上皮内淋巴细胞的IFN-γmRNA表达分别在第6天和第8天显着增加。继发感染后，两种菌株的盲肠扁桃体淋巴细胞之间未见差异，但TK鸡此时肠道小肠上皮内淋巴细胞的IFN-γ转录水平升高。 SC鸡中CD4 +盲肠扁桃体淋巴细胞的选择性耗竭，但CD8 +却没有，导致IFN-γmRNA表达降低，这表明CD4 +细胞是该细胞因子的主要来源。总的来说，这些结果表明局部淋巴细胞应答和IFN-γ的产生受宿主遗传因素的影响。

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