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首页> 外文期刊>Infection and immunity >Production of Basic Fibroblast Growth Factor and Interleukin 6 by Human Smooth Muscle Cells following Infection with Chlamydia pneumoniae
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Production of Basic Fibroblast Growth Factor and Interleukin 6 by Human Smooth Muscle Cells following Infection with Chlamydia pneumoniae

机译：肺炎衣原体感染后人平滑肌细胞产生碱性成纤维细胞生长因子和白介素6

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摘要

Chlamydia pneumoniae infection has been associated with asthma and atherosclerosis. Smooth muscle cells represent host cells for chlamydiae during chronic infection. In this study we demonstrated that C. pneumoniae infection of human smooth muscle cells in vitro increased production of interleukin 6 (IL-6) and basic fibroblast growth factor (bFGF) as shown by reverse transcription-PCR, immunoblotting, and enzyme-linked immunosorbent assay. In contrast, levels of platelet-derived growth factor A-chain mRNA were not affected after infection. The stimulation of bFGF and IL-6 production was most effective when viable chlamydiae were used as inoculum. Furthermore, inhibition of bacterial protein synthesis with chloramphenicol prevented up-regulation of IL-6 and bFGF in infected cells. Addition of IL-6 antibody to infected cultures diminished bFGF expression, indicating involvement of produced IL-6. These findings suggest that chlamydial infection of smooth muscle cells elicits a cytokine response that may contribute to structural remodeling of the airway wall in chronic asthma and to fibrous plaque formation in atherosclerosis.
机译：肺炎衣原体感染与哮喘和动脉粥样硬化有关。平滑肌细胞代表慢性感染过程中衣原体的宿主细胞。在这项研究中，我们证明了体外培养的人类平滑肌细胞肺炎衣原体感染可增加白细胞介素6（IL-6）和碱性成纤维细胞生长因子（bFGF）的产生，如逆转录PCR，免疫印迹和酶联免疫吸附剂所示分析。相反，感染后血小板衍生的生长因子A链mRNA的水平不受影响。当将活的衣原体用作接种物时，对bFGF和IL-6产生的刺激最有效。此外，用氯霉素抑制细菌蛋白质的合成可防止感染细胞中IL-6和bFGF的上调。向感染的培养物中添加IL-6抗体可减少bFGF表达，表明产生的IL-6参与其中。这些发现表明，平滑肌细胞的衣原体感染引起细胞因子反应，其可能有助于慢性哮喘中气道壁的结构重塑和动脉粥样硬化中纤维斑的形成。

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