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Differential Expression of Caveolin-1 in Lipopolysaccharide-Activated Murine Macrophages

机译：Caveolin-1在脂多糖激活的小鼠巨噬细胞中的差异表达。

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Five reciprocal cycles of subtractive hybridization using cDNA generated from fibroblasts with normal lipopolysaccharide (LPS) responsiveness (lps n) and from hyporesponsive (lps d) fibroblasts have led to the finding that caveolin-1 is expressed at markedly higher levels of mRNA inlps d than in lps nfibroblasts. Caveolin-1 message can also be readily detected via reverse transcription-PCR in the RAW264.7 and J774.1 macrophage-like cell lines as well as in primary thioglycolate (TG)-elicited mouse peritoneal macrophages. In RAW264.7 cells, both caveolin-1 mRNA and protein levels are down-regulated by LPS. In TG-elicited C3HeB/FeJ peritoneal macrophages, in contrast, expression of both caveolin-1 protein and mRNA is up-regulated in vitro in response to LPS stimulation. The up-regulation of caveolin-1 protein expression in C3HeB/FeJ peritoneal macrophages can be demonstrated at concentrations as low as 1.0 pg of LPS/ml. However, LPS concentrations approximately 4 orders of magnitude higher (104 pg/ml) were required to stimulate the LPS-hyporesponsive C3H/HeJ mice peritoneal macrophages such that significant caveolin-1 protein up-regulation was detected. Caveolin-1, a principal component of plasmalemmal caveolae, has been reported as a potentially important regulator for signal transduction during cellular stimulation. The results described in this report suggest that caveolin-1 expression may be associated with LPS signaling/internalization.
机译：使用具有正常脂多糖（LPS）响应性（ lps n ）和低反应性（ lps ）的成纤维细胞生成的cDNA，进行消减杂交的五个往复循环> d ）成纤维细胞导致发现， lps d 中Caveolin-1的mRNA表达水平明显高于 lps n 成纤维细胞。也可以通过逆转录-PCR在RAW264.7和J774.1巨噬细胞样细胞系中以及在硫代乙醇酸酯（TG）诱导的小鼠腹膜巨噬细胞中轻易检测到Caveolin-1信息。在RAW264.7细胞中，cavolin-1 mRNA和蛋白质水平均被LPS下调。相反，在TG引起的C3HeB / FeJ腹膜巨噬细胞中，响应LPS刺激，caveolin-1蛋白和mRNA的表达在体外均被上调。 C3HeB / FeJ腹膜巨噬细胞中Caveolin-1蛋白表达的上调可以在低至1.0 pg LPS / ml的浓度下得到证实。但是，刺激LPS低反应性C3H / HeJ小鼠腹膜巨噬细胞需要约4个数量级（10 4 pg / ml）的LPS浓度，从而检测到明显的Caveolin-1蛋白上调。 。据报道，血浆质膜小窝的主要成分Caveolin-1作为细胞刺激过程中信号转导的潜在重要调节剂。该报告中描述的结果表明，caveolin-1表达可能与LPS信号传导/内在化有关。

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