Genetic Immunization of BALB/c mice with a Plasmid Bearing the Gene Coding for a Hybrid Merozoite Surface Protein 1-Hepatitis B Virus Surface Protein Fusion Protects Mice against Lethal Plasmodium chabaudi chabaudi PC1 Infection

Gerhard Wunderlich; Ivan C. Moura; Hernando A. del Portillo

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Genetic Immunization of BALB/c mice with a Plasmid Bearing the Gene Coding for a Hybrid Merozoite Surface Protein 1-Hepatitis B Virus Surface Protein Fusion Protects Mice against Lethal Plasmodium chabaudi chabaudi PC1 Infection

机译：BALB / c小鼠的遗传免疫，其质粒带有杂合子表面蛋白1-乙型肝炎病毒表面蛋白融合基因编码，可保护小鼠免受致死性疟原虫chabaudi chabaudi PC1感染

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The genetic immunization of rodents with a plasmid coding for aPlasmodium chabaudi merozoite surface protein 1 (C terminus)-hepatitis B virus surface fusion protein (pPcMSP1₁₉-HBs) provided protection of mice against subsequent lethal challenge with P. chabaudi chabaudiPC1-infected red blood cells. The percentage of survivor mice was higher in DNA-immunized mice than in animals immunized with a recombinant rPcMSP1₁₉– glutathioneS-transferase fusion protein administered in Freund adjuvant. In all mice immunized with the pPcMSP1₁₉-HBs, a Th1-specific response, including the production of anti-MSP1₁₉-specific immunoglobulins predominantly of the immunoglobulin G2a subtype and reacting almost exclusively against discontinuous epitopes, was elicited. The coinjection of Th1-type cytokine-expressing plasmids (gamma interferon, interleukin-2, and granulocyte-macrophage colony-stimulating factor) mostly abolished protection and boosting of MSP1₁₉-specific antibodies. The inclusion of a lymph node-targeting signal did not significantly increase protection. These data provide further evidence that MSP1₁₉-HBs DNA constructs might be useful as components of a genetic vaccine against the asexual blood stages ofPlasmodium.

机译：用编码 chabaudi 裂殖子表面蛋白1（C末端）-乙型肝炎病毒表面融合蛋白（pPcMSP1 _{19 -HBs）的质粒对啮齿类动物进行遗传免疫小鼠抵抗随后的 P致死性攻击。 chabaudi chabaudi PC1感染的红细胞。 DNA免疫小鼠中存活小鼠的比例高于弗氏佐剂中重组rPcMSP1 _{19 -谷胱甘肽 S -转移酶融合蛋白免疫的动物。在用pPcMSP1 _{19 -HBs免疫的所有小鼠中，Th1特异性应答，包括主要是免疫球蛋白G2a亚型的抗MSP1 _{19 特异性免疫球蛋白的产生和反应引发了几乎专门针对不连续表位的抗原。 Th1型细胞因子表达质粒（γ干扰素，白介素2和粒细胞巨噬细胞集落刺激因子）的共同注射在很大程度上消除了对MSP1 _{19 特异性抗体的保护和增强作用。包含淋巴结靶向信号并未显着增加保护作用。这些数据提供了进一步的证据，证明MSP1 _{19 -HBs DNA构建体可用作针对 Plasmodium 的无性血液阶段的基因疫苗的组成部分。}}}}}} 展开▼

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《Infection and immunity》 |2000年第10期|共7页

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Gerhard Wunderlich; Ivan C. Moura; Hernando A. del Portillo;
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1. Genetic Immunization of BALB/c mice with a Plasmid Bearing the Gene Coding for a Hybrid Merozoite Surface Protein 1-Hepatitis B Virus Surface Protein Fusion Protects Mice against Lethal Plasmodium chabaudi chabaudi PC1 Infection [J] . Gerhard Wunderlich, Ivan C. Moura, Hernando A. del Portillo Infection and immunity . 2000,第10期

机译：BALB / c小鼠的遗传免疫，其质粒带有杂合子表面蛋白1-乙型肝炎病毒表面蛋白融合基因编码，可保护小鼠免受致死性疟原虫chabaudi chabaudi PC1感染

2. A Bicistronic DNA Vaccine Containing Apical Membrane Antigen 1 and Merozoite Surface Protein 4/5 Can Prime Humoral and Cellular Immune Responses and Partially Protect Mice against Virulent Plasmodium chabaudi adami DS Malaria [J] . A. Rainczuk, T. Scorza, T. W. Spithill, Infection and immunity . 2004,第10期

机译：包含顶膜抗原1和裂殖子表面蛋白4/5的双顺反子DNA疫苗可以引发体液和细胞的免疫反应，并部分保护小鼠免于感染Chabaudi adami DS恶性疟原虫。

3. Gene gun-based co-immunization of merozoite surface protein-1 cDNA with IL-12 expression plasmid confers protection against lethal Plasmodium yoelii in A/J mice [J] . Sakai T, Hisaeda H, Nakano Y, Vaccine . 2003,第13a14期

机译：基于基因枪的裂殖子表面蛋白-1 cDNA与IL-12表达质粒的共免疫可保护A / J小鼠免于致命的约氏疟原虫

4. Prime boost vaccination with pSG2 plasmid and MVA each recombinant for a MAP fusion protein attenuates pre-existing MAP infection in C57/BL6 mice [C] . Bull TJ, Sidi-Boumedine S, Cen-Si, International Colloquium on Paratuberculosis . 2007

机译：用PSG2质粒和MVA的初始促进疫苗接种，每种重组地图融合蛋白在C57 / BL6小鼠中衰减预先存在的地图感染

5. Expression and characterization of the 42kDa carboxyl-terminal processing fragment of Plasmodium falciparum merozoite surface protein-1 (PfMSP-1(42)) in silkworm larvae using Bombyx mori nuclear polyhedrosis virus. [D] . Pang, Lap-yin. 2000

机译：使用家蚕核多角体病毒在家蚕幼虫中恶性疟原虫裂殖子表面蛋白-1（PfMSP-1（42））的42kDa羧基末端加工片段的表达和鉴定。

6. Genetic Immunization of BALB/c mice with a Plasmid Bearing the Gene Coding for a Hybrid Merozoite Surface Protein 1-Hepatitis B Virus Surface Protein Fusion Protects Mice against Lethal Plasmodium chabaudi chabaudi PC1 Infection [O] . Gerhard Wunderlich, Ivan C. Moura, Hernando A. del Portillo 2000

机译：BALB / c小鼠的遗传免疫其质粒带有杂合子表面蛋白1-乙型肝炎病毒表面蛋白融合基因编码可保护小鼠免受致死性疟原虫chabaudi chabaudi PC1感染

7. Genetic Immunization of BALB/c mice with a Plasmid Bearing the Gene Coding for a Hybrid Merozoite Surface Protein 1-Hepatitis B Virus Surface Protein Fusion Protects Mice against Lethal Plasmodium chabaudi chabaudi PC1 Infection [O] . Wunderlich, Gerhard, Moura, Ivan C., del Portillo, Hernando A. 2000

机译：BALB / c小鼠的遗传免疫，其质粒带有杂合子表面蛋白1-乙型肝炎病毒表面蛋白融合基因编码，可保护小鼠免受致死性疟原虫chabaudi chabaudi PC1感染

8. Mice Immunized with a Dengue Type 2 Virus E and NS1 Fusion Protein Made inEscherichia Coli are Protected against Lethal Dengue Virus Infection [R] . Ivastava, A. K., Putnak, J. R., Warren, R. L., 1995

机译：用登革热2型病毒免疫的小鼠E和大肠杆菌中的Ns1融合蛋白受到致死登革病毒感染的保护

1. 009重组约氏疟原虫裂殖子表面蛋白 4/5对小鼠致死性攻击的免疫保护 [J] . . 国际医学寄生虫病杂志 . 2002,第001期

1. A FUSION PROTEIN MALARIA VACCINE CONSISTING OF PLASMODIUM FALCIPARUM MEROZOITE SURFACE PROTEIN 1 AND PLASMODIUM FALCIPARUM MEROZOITE SURFACE PROTEIN 3 [P] . 外国专利： IN282172B . 2017-04-07

机译：恶性疟原虫裂殖子表面蛋白1和恶性疟原虫裂殖子表面蛋白3的融合蛋白疟疾疫苗。

2. USE OF FUSION PROTEIN CONSISTING OF FRAGMENTS OF PLASMODIUM FALCIPARUM MEROZOITE SURFACE PROTEIN 1 AND PLASMODIUM FALCIPARUM MEROZOITE SURFACE PROTEIN 3 AS A MALARIA VACCINE [P] . 外国专利： IN2008DE01737A . 2010-04-23

机译：恶性疟原虫裂殖子表面蛋白1和恶性疟原虫裂殖子表面蛋白3片段的融合蛋白作为疟疾疫苗的应用

3. Vaccine comprises chimeric malaria proteins derived from Plasmodium falciparum genetically coupled to Plasmodium falcinarum Merozoite surface protein 3 [P] . 外国专利： DK200201741A . 2003-09-16

机译：疫苗包含源自恶性疟原虫的嵌合疟疾蛋白，该蛋白通过遗传与恶性疟原虫裂殖子表面蛋白3遗传偶联

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Genetic Immunization of BALB/c mice with a Plasmid Bearing the Gene Coding for a Hybrid Merozoite Surface Protein 1-Hepatitis B Virus Surface Protein Fusion Protects Mice against Lethal Plasmodium chabaudi chabaudi PC1 Infection

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