• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Infection and immunity >Mucosal Immune Responses and Protection against Tetanus Toxin after Intranasal Immunization with RecombinantLactobacillus plantarum
【24h】

Mucosal Immune Responses and Protection against Tetanus Toxin after Intranasal Immunization with RecombinantLactobacillus plantarum

机译：重组植物乳杆菌鼻内免疫后黏膜免疫反应及对破伤风毒素的保护

获取原文
       
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The use of live microorganisms as an antigen delivery system is an effective means to elicit local immune responses and thus represents a promising strategy for mucosal vaccination. In this respect, lactic acid bacteria represent an original and attractive approach, as they are safe organisms that are used as food starters and probiotics. To determine whether an immune response could be elicited by intranasal delivery of recombinant lactobacilli, a Lactobacillus plantarum strain of human origin (NCIMB8826) was selected as the expression host. Cytoplasmic production of the 47-kDa fragment C of tetanus toxin (TTFC) was achieved at different levels depending on the plasmid construct. All recombinant strains proved to be immunogenic by the intranasal route in mice and able to elicit very high systemic immunoglobulin G (IgG1, IgG2b, and IgG2a) responses which correlated to the antigen dose. No significant differences in enzyme-linked immunosorbent assay IgG titers were observed when mice were immunized with live or mitomycin C-treated recombinant lactobacilli. Nevertheless, protection against the lethal effect of tetanus toxin was obtained only with the strains producing the highest dose of antigen and was greater following immunization with live bacteria. Significant TTFC-specific mucosal IgA responses were measured in bronchoalveolar lavage fluids, and antigen-specific T-cell responses were detected in cervical lymph nodes, both responses being higher in mice receiving a double dose of bacteria (at a 24-h interval) at each administration. These results demonstrate that recombinant lactobacilli can induce specific humoral (protective) and mucosal antibodies and cellular immune response against protective antigens upon nasal administration.
机译：使用活微生物作为抗原递送系统是引发局部免疫应答的有效手段，因此代表了粘膜疫苗接种的有前途的策略。在这方面，乳酸菌代表了一种新颖而诱人的方法，因为它们是用作食品发酵剂和益生菌的安全生物。为了确定鼻内递送重组乳杆菌是否可以引起免疫反应，选择了人类来源的植物乳杆菌（NCIMB8826）作为表达宿主。破伤风毒素（TTFC）的47 kDa片段C的细胞质产生取决于质粒构建体的不同水平。事实证明，所有重组株均通过小鼠鼻内途径具有免疫原性，并能够引起与抗原剂量相关的很高的全身免疫球蛋白G（IgG1，IgG2b和IgG2a）应答。当用活的或丝裂霉素C处理的重组乳杆菌免疫小鼠时，在酶联免疫吸附法IgG滴度上没有观察到显着差异。然而，仅在产生最高剂量抗原的菌株中获得针对破伤风毒素致死作用的保护，并且在用活细菌免疫后更强。在支气管肺泡灌洗液中检测到了显着的TTFC特异性粘膜IgA反应，在宫颈淋巴结中检测到了抗原特异性T细胞反应，在接受双倍剂量细菌（间隔24小时）的小鼠中，两种反应均较高。每个主管部门。这些结果证明，经鼻施用后，重组乳杆菌可诱导特异性的体液（保护性）和粘膜抗体以及针对保护性抗原的细胞免疫应答。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号