• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Infection and immunity >Stable Transfection of the BovineNRAMP1 Gene into Murine RAW264.7 Cells: Effect onBrucella abortus Survival
【24h】

Stable Transfection of the BovineNRAMP1 Gene into Murine RAW264.7 Cells: Effect onBrucella abortus Survival

机译：牛NRAMP1基因稳定转染到小鼠RAW264.7细胞中：对流产布鲁氏菌存活的影响

获取原文
       
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Genetically based natural resistance to brucellosis in cattle provides for novel strategies to control zoonotic diseases. BovineNRAMP1, the homologue of a murine gene (Bcg), has been identified as a major candidate for controlling the in vivo resistant phenotype. We developed an in vitro model for expression of resistance- and susceptibility-associated alleles of bovine NRAMP1 as stable transgenes under the regulatory control of the bovineNRAMP1 promoter in the murine RAW264.7 macrophage cell line (Bcg s) to analyze the regulation of the NRAMP1 gene and its role in macrophage function. We demonstrated that the 5′-flanking region of bovineNRAMP1, despite the lack of TATA and CAAT boxes, has a functional promoter capable of driving the expression of a transgene in murine macrophages. A polymorphism within a microsatellite in the 3′ untranslated region critically affects the expression of bovineNRAMP1 and the control of in vitro replication ofBrucella abortus but not Salmonella enterica serovar Dublin. We did not observe any differences in the production of NO by resting or gamma interferon (IFN-γ)- and IFN-γ–lipopolysaccharide (LPS)-treated transfected cell lines, yet the resistant transfected cell lines produced significantly less NO than other cell lines, following stimulation with LPS at 24 and 48 h.
机译：牛对布鲁氏菌病的基于遗传的天然抗性为控制人畜共患病提供了新的策略。牛 NRAMP1 是鼠基因（ Bcg ）的同源物，已被确定为控制体内抗性表型的主要候选对象。我们开发了一个体外模型，用于在牛RAW264牛 NRAMP1 启动子的调控下，将牛 NRAMP1 的抗性和敏感性相关等位基因表达为稳定的转基因。 7个巨噬细胞系（ Bcg s ）分析 NRAMP1 基因的调控及其在巨噬细胞功能中的作用。我们证明牛 NRAMP1 的5'侧翼区域尽管缺乏TATA和CAAT框，但具有能够驱动鼠巨噬细胞中转基因表达的功能性启动子。 3'非翻译区微卫星内的多态性严重影响牛 NRAMP1 的表达以及流产 Brucella abortus 的复制复制的控制，而不影响 Salmonella enterica < / em>都柏林的serovar。我们没有观察到静息或经γ-干扰素（IFN-γ）和IFN-γ-脂多糖（LPS）处理的转染细胞系在NO产生方面的任何差异，但抗性转染细胞系产生的NO明显少于其他细胞在24和48 h用LPS刺激后的品系。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号