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首页> 外文期刊>Infection and immunity >Human antibody response to a group B serotype 2a meningococcal vaccine determined by immunoblotting.
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Human antibody response to a group B serotype 2a meningococcal vaccine determined by immunoblotting.

机译：通过免疫印迹测定对B组血清型2a脑膜炎球菌疫苗的人抗体反应。

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The antibody response of 30 volunteers vaccinated with a complex of group B polysaccharide and outer membrane vesicles (OMV) from serotype 2a Neisseria meningitidis and of 3 individuals who received a placebo vaccine was determined by immunoblotting. OMV were separated by sodium dodecyl sulfate-gel electrophoresis and electrotransferred to nitrocellulose filters. Binding of immunoglobulin G (IgG), IgA, and IgM antibodies in the human sera to OMV components was detected with class-specific peroxidase-conjugated antibodies. The immunoblotting results were also related to the bactericidal activity of the sera and the meningococcal carrier status of the volunteers. Before vaccination weakly reactive bands in the molecular weight range of 140,000 to 10,000 were observed on the blots. Sera from carriers showed more marked bands. Individual patterns of increased reactivity were seen 6 weeks after vaccination. The main immunoreactive components of OMV corresponded to a molecular weight of 43,000 (class 1 protein), 30,000 (class 5 proteins), and 22,000. IgG antibodies in postvaccination sera of high bactericidal titers showed distinct binding to the 43,000-molecular-weight antigen. Meningococcal carriers had antibodies against an antigen of 22,000 molecular weight; in polyacrylamide gels this component did not stain with Coomassie brilliant blue or silver. The marked binding of IgG antibodies to the class 5 proteins decreased considerably between weeks 6 and 25 after vaccination. Periodate oxidation of OMV abolished the binding of IgG antibodies to the class 5 proteins, whereas the antigenicity of the 43,000-molecular-weight (class 1 protein) and 22,000-molecular-weight antigens was unaffected.
机译：通过免疫印迹法确定了30名接种了B型多糖和脑膜炎奈瑟氏球菌外膜囊泡复合物的复合物的30名志愿者的免疫应答，以及3名接受安慰剂疫苗的个体的免疫应答。通过十二烷基硫酸钠-凝胶电泳分离OMV，并将其电转移到硝酸纤维素滤膜上。用类特异性过氧化物酶偶联的抗体检测了人血清中免疫球蛋白G（IgG），IgA和IgM抗体与OMV成分的结合。免疫印迹结果还与志愿者的血清杀菌活性和脑膜炎球菌携带者状况有关。接种疫苗之前，在印迹上观察到分子量范围为140,000至10,000的弱反应带。携带者的血清显示出更明显的条带。接种后6周，观察到个体反应性增加。 OMV的主要免疫反应性成分分别对应于43,000（1类蛋白质），30,000（5类蛋白质）和22,000的分子量。高杀菌效价的疫苗接种后血清中的IgG抗体显示出与43,000分子量抗原的独特结合。脑膜炎球菌载体具有抗22,000分子量抗原的抗体;在聚丙烯酰胺凝胶中，该组分不会被考马斯亮蓝或银染。疫苗接种后第6周到25周之间，IgG抗体与5类蛋白质的显着结合大大降低。 OMV的高碘酸氧化消除了IgG抗体与5类蛋白的结合，而43,000分子量（1类蛋白）和22,000分子量抗原的抗原性不受影响。

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