...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Infection and immunity >Resistance to pesticin, storage of iron, and invasion of HeLa cells by Yersiniae.
【24h】

Resistance to pesticin, storage of iron, and invasion of HeLa cells by Yersiniae.

机译：耶尔森氏菌对农药的抗性，铁的储存和对HeLa细胞的侵袭。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The independent abilities of Yersinia pestis to absorb exogenous pigments including hemin and Congo red (Pgm+) and to produce the bacteriocin pesticin with genetically linked invasive enzymes (Pst+) are established virulence factors of the species. Pst- Pgm+ strains of Y. pestis are sensitive to pesticin (Psts), and mutation of these isolates to pesticin resistance (Pstr) is known to result in concomitant conversion to Pgm-. Wild-type cells of Yersinia pseudotuberculosis and Yersinia enterocolitica are Pgm- but may be Psts; mutation of the latter to Pstr also results in avirulence. In this study, typical Pgm- mutants of Y. pestis exhibited a dramatic nutritional requirement at 37 degrees C but not 26 degrees C for iron which could be fulfilled by either Fe3+ or hemin. Iron privation of Pgm- yersiniae resulted in formation of osmotically stable spheroplasts similar to those previously observed after exposure of Psts bacteria to pesticin. At 37 degrees C, Pgm+ organisms rapidly overgrew initially predominant Pgm- populations in iron-deficient medium. However, Pgm-isolates could undergo a second mutation that permitted successful competition with Pgm+ cells in this environment. The mutation to Pstr in Y. pseudotuberculosis and Y. enterocolitica did not promote a similar requirement for iron but rather prevented these organisms from penetrating HeLa cells. The ability to invade these nonprofessional phagocytes was not shared by Pgm+ or Pgm- cells of Y. pestis.
机译：鼠疫耶尔森氏菌吸收包括血红素和刚果红（Pgm +）在内的外源色素以及与遗传相关的侵入性酶（Pst +）产生细菌素杀虫菌素的独立能力是该物种已确定的毒力因子。鼠疫耶尔森氏菌的Pst-Pgm +菌株对瘟疫素（Psts）敏感，并且已知这些分离株突变为瘟疫素抗性（Pstr）会导致同时转化为Pgm-。假结核耶尔森菌和小肠结肠炎耶尔森菌的野生型细胞均为Pgm-，但可能为Psts。后者突变为Pstr也会导致无毒力。在这项研究中，典型的鼠疫耶尔森氏菌Pgm-突变体在37℃时对铁具有显着的营养需求，而在26℃时对铁的需求不明显，这可以通过Fe3 +或血红素实现。铁缺乏鼠李斯特菌导致形成了渗透稳定的原生质球，类似于先前将Psts细菌暴露于农药中所观察到的。在37摄氏度时，Pgm +生物在铁缺乏的培养基中迅速过量繁殖最初占优势的Pgm-种群。但是，Pgm分离物可能会经历第二次突变，从而允许在这种环境下与Pgm +细胞成功竞争。假结核耶尔森氏菌和小肠结肠炎耶尔森氏菌中Pstr的突变并未促进对铁的类似需求，但阻止了这些生物体穿透HeLa细胞。鼠疫耶尔森氏菌的Pgm +或Pgm-细胞没有共享侵袭这些非专业吞噬细胞的能力。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号