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首页> 外文期刊>Infection and immunity >Intestinal mucus gel and secretory antibody are barriers to Campylobacter jejuni adherence to INT 407 cells.
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Intestinal mucus gel and secretory antibody are barriers to Campylobacter jejuni adherence to INT 407 cells.

机译：肠道粘液凝胶和分泌抗体是空肠弯曲杆菌粘附至INT 407细胞的障碍。

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摘要

An in vitro mucus assay was developed to study the role of mucus gel and secretory immunoglobulin A (sIgA) in preventing attachment of Campylobacter jejuni to INT 407 cells. An overlay of rabbit small intestinal mucus was found to impede the attachment of C. jejuni to a monolayer of INT 407 cells. Mucus from rabbits previously colonized with C. jejuni was found to completely inhibit bacterial adherence to the underlying cells. Anti-Campylobacter sIgA was readily detected in mucus samples from previously exposed rabbits and was responsible for eliminating bacterial adherence to the INT 407 cells. This was shown by loss of inhibition after mucus absorption with Campylobacter cells. sIgA-containing mucus caused aggregation of the C. jejuni cells within the mucus layer of the assay system. Nonimmune mucus and sIgA alone were unable to cause bacterial aggregation, suggesting a cooperative role for mucus and sIgA. Antibodies responsible for adhesion inhibition were cross-reactive among several Campylobacter strains and were not directed solely against flagellar antigens.
机译：开发了一种体外粘液测定法，以研究粘液凝胶和分泌性免疫球蛋白A（sIgA）在防止空肠弯曲杆菌附着至INT 407细胞中的作用。发现兔小肠粘液的覆盖物阻碍空肠弯曲杆菌附着至单层INT 407细胞。发现先前曾在空肠弯曲杆菌中定殖过的兔子的粘液可完全抑制细菌对下层细胞的粘附。在先前暴露过的兔子的粘液样本中很容易检测到抗弯曲杆菌sIgA，它可消除细菌对INT 407细胞的粘附。这通过弯曲杆菌细胞吸收粘液后抑制作用的丧失来表明。含有sIgA的黏液会导致空肠弯曲杆菌细胞在测定系统黏液层内聚集。单独的非免疫粘液和sIgA不能引起细菌聚集，表明粘液和sIgA具有协同作用。负责粘附抑制的抗体在几种弯曲杆菌菌株之间具有交叉反应性，并且不仅仅针对鞭毛抗原。

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