• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Infection and immunity >Characterization of lectinlike surface components on Capnocytophaga ochracea ATCC 33596 that mediate coaggregation with gram-positive oral bacteria.
【24h】

Characterization of lectinlike surface components on Capnocytophaga ochracea ATCC 33596 that mediate coaggregation with gram-positive oral bacteria.

机译：Capnocytophaga ochracea ATCC 33596上凝集素样表面成分的表征，该成分介导与革兰氏阳性口腔细菌的共聚集。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The interactions between Capnocytophaga ochracea ATCC 33596 and Streptococcus sanguis H1, Actinomyces naeslundii PK984, or Actinomyces israelii PK16 are dependent on specific recognitions between heat-sensitive adhesins on C. ochracea and heat-stable structures (probably carbohydrate-containing receptors) on the surfaces of these gram-positive coaggregation partners. The coaggregation of C. ochracea with each of these three organisms was inhibited by L-rhamnose and D-fucose and to a lesser extent by beta-methyl-galactoside. The reaction with S. sanguis was the most sensitive, while the coaggregation with A. israelii was the least sensitive and was only partially inhibited by each of the sugars that were considered to be effective inhibitors. A more effective inhibition of the coaggregation between C. ochracea and A. israelii was achieved by adding a combination of the 6-deoxysugars and N-acetylneuraminic acid. To further characterize the coaggregations, naturally occurring coaggregation-defective (Cog-) mutants of C. ochracea were obtained from several different selections. Three phenotypically distinct groups of mutants were were isolated. Type 1 mutants failed to coaggregate with S. sanguis only. Type 2 mutants lost ability to interact with both S. sanguis and A. naeslundii. Type 3 mutants failed to coaggregate with all three coaggregation partners. Characterization of the Cog- mutants by sugar inhibition studies made it possible to distinguish three classes of adhesin activity.
机译：Capnocytophaga ochracea ATCC 33596和链球菌H1，Naeslundii PK984或以色列放线菌PK16之间的相互作用取决于对C. ochracea的热敏粘附素和表面热稳定结构（可能含有碳水化合物的受体）的特异性识别。这些革兰氏阳性聚集伙伴。 L-鼠李糖和D-岩藻糖抑制豆衣藻与这三种生物的共聚，而β-甲基-半乳糖苷抑制程度较小。与sanguis的反应是最敏感的，而与以色列链霉菌的共聚集是最不敏感的，并且仅被认为是有效抑制剂的每种糖部分抑制。通过加入6-脱氧糖和N-乙酰神经氨酸的组合，可以更有效地抑制曲藻和以色列曲霉的共聚集。为了进一步表征共聚集体，从几种不同的选择中获得了天然存在的草梭菌的共聚集缺陷型（Cog-）突变体。分离了三个表型不同的突变体组。 1型突变体仅与S. sanguis不能聚集。 2型突变体失去了与桑氏沙门氏菌和内氏拟南芥相互作用的能力。 3型突变体未能与所有三个共聚伙伴共聚。通过糖抑制研究表征Cog-突变体使得区分三类粘附素活性成为可能。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号