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首页> 外文期刊>Infection and immunity >Effectiveness of natural and synthetic complexes of porin and O polysaccharide as vaccines against Brucella abortus in mice.
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Effectiveness of natural and synthetic complexes of porin and O polysaccharide as vaccines against Brucella abortus in mice.

机译：天然和合成的孔蛋白和O多糖复合物作为抗流产布鲁氏菌疫苗的小鼠的功效。

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A single vaccination of mice with a complex of porin and smooth lipopolysaccharide (porin-S-LPS) extracted from virulent Brucella abortus 2308 provided significant protection (P less than 0.01 to P less than 0.001) against challenge with the same strain, equivalent to that achieved by vaccination with living attenuated B. abortus 19. The porin-S-LPS vaccine given without adjuvant or in several adjuvants (trehalose dimycolate and muramyl dipeptide; the pluronic polymer L-121 and muramyl dipeptide; or complexed with Quil A in immunostimulating complexes) provided equivalent protection. In contrast, one vaccination with porin complexed with rough LPS (porin-R-LPS) from a rough mutant of strain 2308 provided no protection with any adjuvant tested. In one experiment, two inoculations with the porin-R-LPS resulted in a low level of protection, probably owing to priming of the animals for production of O-polysaccharide-specific antibodies. However, one vaccination with rough-strain porin covalently bound to purified O polysaccharide conferred protection equal to that obtained with natural complexes of porin-S-LPS or with living strain 19. A synthetic vaccine containing long chains of O polysaccharide was more effective than one prepared with short chains. Protective vaccines caused the formation of increased concentrations of circulating O-polysaccharide-specific antibodies, although there were individual exceptions to the quantitative association between O-polysaccharide-specific antibodies and protection. Antibodies specific for porin or R-LPS were found in negligible quantities in vaccinated mice. These results provide additional evidence that the O polysaccharide will constitute an essential component of an effective subcellular vaccine against B. abortus and that O-polysaccharide-specific antibodies play an important role in protective immunity in brucellosis.
机译：小鼠单次接种具有毒性的流产布鲁氏菌2308提取的孔蛋白和平滑脂多糖（porin-S-LPS）的复合物可对相同菌株的攻击提供显着保护（P小于0.01至P小于0.001），相当于19.用减毒活的流产双歧杆菌进行疫苗接种可达到的目的。Porin-S-LPS疫苗可不加佐剂或以几种佐剂（海藻糖二甲酸酯和双酰二肽;普卢尼克聚合物L-121和双酰二肽;或在免疫刺激复合物中与Quil A复合）给予）提供了等效的保护。相反，用与来自菌株2308的粗糙突变体的粗糙LPS（孔蛋白-R-LPS）复合的孔蛋白进行的一次疫苗接种没有用任何测试的佐剂提供保护。在一个实验中，两次接种孔蛋白-R-LPS导致的保护水平很低，这可能是由于引发动物产生O-多糖特异性抗体所致。但是，用共价键结合到纯化的O多糖的粗菌株Porin进行的一次疫苗接种可提供与用Porin-S-LPS天然复合物或活菌株19所获得的疫苗相同的保护作用。含有O多糖长链的合成疫苗比一种疫苗更有效。用短链准备的。保护性疫苗导致形成的O-多糖特异性循环抗体浓度升高，尽管O-多糖特异性抗体与保护之间的定量关联存在个别例外。在疫苗接种的小鼠中发现对孔蛋白或R-LPS特异的抗体数量可忽略不计。这些结果提供了另外的证据，即O多糖将构成抗流产芽孢杆菌的有效亚细胞疫苗的重要组成部分，并且O多糖特异性抗体在布鲁氏菌病的保护性免疫中起重要作用。

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