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首页> 外文期刊>Infection and immunity >Identification of leishmanial antigens in the sera of patients with American visceral leishmaniasis.
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Identification of leishmanial antigens in the sera of patients with American visceral leishmaniasis.

机译：美国内脏利什曼病患者血清中利什曼抗原的鉴定。

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Circulating immune complexes are present in the sera of patients with visceral leishmaniasis caused by Leishmania donovani chagasi. In order to determine whether these complexes contain parasite antigens, sera were collected from Brazilian patients with visceral leishmaniasis and from hospitalized control subjects with other diagnoses. High-molecular-weight complexes were precipitated from pooled sera with 2.5% polyethylene glycol. Approximately 140-fold-more protein was precipitated from patient sera than from control sera; 12% of the total patient serum protein was precipitated. Patient serum precipitates contained immunoglobulins G (525 mg/dl), M (27 mg/dl), and A (8 mg/dl). Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of the patient serum precipitates revealed multiple bands, including a prominent band at 70 kilodaltons, that were not seen in precipitates of control sera. The 70-kilodalton band was recognized by human and hamster sera with antileishmanial antibodies, but not by control sera. Finally, immunization of BALB/c mice with the high-molecular-weight precipitates from patients elicited antileishmanial antibodies against L. donovani chagasi antigens as detected by enzyme-linked immunosorbent assay and Western blot (immunoblot) assay. In summary, sera of patients with American visceral leishmaniasis were found to have high-molecular-weight complexes that contained one or more parasite antigens. These complexes may play a role in the immunology of the disease, and detection of circulating parasite antigens has potential diagnostic importance.
机译：循环免疫复合物存在于由Leishmania donovani chagasi引起的内脏利什曼病患者的血清中。为了确定这些复合物是否含有寄生虫抗原，从患有内脏利什曼病的巴西患者和其他诊断出的住院对照患者中收集血清。用2.5％聚乙二醇从合并的血清中沉淀出高分子量复合物。从患者血清中沉淀出的蛋白质比从对照血清中沉淀出的蛋白质多出约140倍。全部患者血清蛋白中有12％沉淀出来。患者血清沉淀物包含免疫球蛋白G（525 mg / dl），M（27 mg / dl）和A（8 mg / dl）。患者血清沉淀物的十二烷基硫酸钠-聚丙烯酰胺凝胶电泳显示出多个条带，包括在70道尔顿处的一条显着条带，在对照血清的沉淀物中看不到。人和仓鼠血清中含有抗动物抗性抗体，可识别70千达尔顿的条带，而对照血清则不能。最后，用高分子量沉淀物对患者的BALB / c小鼠进行免疫接种，可通过抗酶联免疫吸附法和Western blot（免疫印迹）法检测出抗南美白对虾抗原的抗纤毛虫抗体。总之，发现患有美国内脏利什曼病的患者的血清具有包含一种或多种寄生虫抗原的高分子量复合物。这些复合物可能在疾病的免疫学中发挥作用，循环中寄生虫抗原的检测具有潜在的诊断重要性。

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