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首页> 外文期刊>Infection and immunity >Identification of a pathogenic isolate-specific 30,000-Mr antigen of Entamoeba histolytica by using a monoclonal antibody.
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Identification of a pathogenic isolate-specific 30,000-Mr antigen of Entamoeba histolytica by using a monoclonal antibody.

机译：通过使用单克隆抗体鉴定溶组织变形杆菌的病原性分离物特异性30,000-Mr抗原。

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摘要

A monoclonal antibody (MAb) produced against trophozoites of Entamoeba histolytica strain HM-1:IMSS, reacted with all of 42 isolates and 4 clones showing pathogenic zymodeme (Z) patterns, i.e., Z-II, Z-II alpha-, Z-II (glucose phosphate isomerase: gamma +), Z-VII, Z-VII (glucose phosphate isomerase: alpha lack, gamma +), Z-XI, Z-XIV, and Z-XIX, regardless of culture conditions, geographical origins, or host symptoms in an indirect fluorescence antibody test. In contrast, the MAb failed to react with 14 isolates possessing nonpathogenic zymodemes Z-I and Z-VIII and did not react with other enteric protozoan parasites, such as E. histolytica-like Laredo, Entamoeba hartmanni, Entamoeba coli, Endolimax nana, Dientamoeba fragilis, Trichomonas hominis, and Giardia lamblia. Western immunoblotting analysis showed that the molecular weight of the antigenic component recognized by the MAb was exclusively 30,000 in pathogenic isolates of different zymodemes. These results suggest that the 30,000-molecular-weight antigen is a marker of pathogenic isolates and that the indirect fluorescent-antibody test with the MAb is useful for the accurate discrimination of pathogenic amebae.
机译：产生了一种针对抗组织变形杆菌Entrooeba histolytica菌株HM-1：IMSS的滋养体的单克隆抗体（MAb），与所有42个分离株和4个显示病原体（Z）模式的克隆进行反应，即Z-II，Z-IIα-，Z II（葡萄糖磷酸异构酶：γ+），Z-VII，Z-VII（葡萄糖磷酸异构酶：α缺乏，γ+），Z-XI，Z-XIV和Z-XIX，无论培养条件，地理位置如何，间接荧光抗体测试中出现宿主症状。相反，MAb不能与14种具有非致病性zymodes ZI和Z-VIII的分离物反应，并且不与其他肠原生动物寄生虫反应，例如像溶血性大肠杆菌一样的Laredo，Entamoeba hartmanni，Entamoeba coli，Endolimax nana，Dientamoeba fragilis，毛滴虫和贾第鞭毛虫。 Western免疫印迹分析表明，在不同zymodes的致病性分离物中，MAb识别的抗原成分的分子量仅为30,000。这些结果表明，分子量为30,000的抗原是病原分离株的标志物，使用MAb进行的间接荧光抗体测试可用于准确区分病原性变形虫。

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