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首页> 外文期刊>Infection and immunity >Comparison of receptors required for entry of Leishmania major amastigotes into macrophages.
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Comparison of receptors required for entry of Leishmania major amastigotes into macrophages.

机译：比较利什曼原虫主要变形虫进入巨噬细胞所需的受体。

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We investigated the mechanisms of entry of amastigotes of Leishmania major from two different sources into macrophages by comparing their use of the Fc receptor (FcR), complement receptor type 3 (CR3), and mannose-fucose receptor (MFR). Amastigotes were obtained from BALB/c mice and SCID mice. FcR involvement was examined by opsonizing L. major with parasite-specific immunoglobulin G (IgG). Antiparasite IgG did not alter the uptake of amastigotes from BALB/c mice since these amastigotes had antibody bound to their surface: IgG1 was the most predominant antibody, followed by IgG2b, IgM, and IgG2a. However, opsonization with antiparasite IgG enhanced the entry of amastigotes that lacked antibody on their surface, namely, amastigotes obtained from SCID mice or from macrophages infected in vitro. These results indicate that the FcR is important for amastigote entry into macrophages. Down-modulation of FcRs onto immune complexes, however, did not reduce the entry of amastigotes containing surface-bound IgG into macrophages. Monoclonal antibodies against the CR3 inhibited the entry of amastigotes from either BALB/c or SCID mice into J774A.1 macrophage-like cells. Simultaneous blocking of FcR and CR3 further increased the inhibition of phagocytosis. Treatment of macrophages with soluble mannan or down-modulating the MFR onto mannan-coated coverslips had no effect on the entry of amastigotes from BALB/c or SCID mice. Thus, the MFR does not appear to be used by amastigotes of L. major. We show that ingestion of amastigotes appears to occur primarily through the FcR and CR3; however, additional receptors may also participate in the uptake of amastigotes.
机译：我们通过比较它们对Fc受体（FcR），3型补体受体（CR3）和甘露糖-岩藻糖受体（MFR）的使用，研究了来自两种不同来源的利什曼原虫的变形虫进入巨噬细胞的机制。从BALB / c小鼠和SCID小鼠获得变形虫。 FcR的参与通过用寄生虫特异性免疫球蛋白G（IgG）调理大麦芽孢杆菌来检查。抗寄生虫IgG不会改变BALB / c小鼠对amastigotes的摄取，因为这些amastigotes的表面结合了抗体：IgG1是最主要的抗体，其次是IgG2b，IgM和IgG2a。但是，用抗寄生虫IgG调理作用可增强表面上缺乏抗体的变形虫的进入，即从SCID小鼠或体外感染的巨噬细胞获得的变形虫。这些结果表明，FcR对于a蛇毒进入巨噬细胞是重要的。但是，将FcR下调到免疫复合物上并不能减少含有表面结合的IgG的amastigotes进入巨噬细胞。针对CR3的单克隆抗体抑制了Amastigotes从BALB / c或SCID小鼠进入J774A.1巨噬细胞样细胞。同时阻断FcR和CR3进一步增强了对吞噬作用的抑制作用。用可溶性甘露聚糖处理巨噬细胞或将MFR下调至甘露聚糖包被的盖玻片上对从BALB / c或SCID小鼠进入的变形虫没有影响。因此，MFR似乎不被大乳利什曼原虫使用。我们显示摄入amastigotes似乎主要通过FcR和CR3发生。但是，其他受体也可能参与了羊膜的摄取。

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