• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Infection and immunity >Requirements for CD4+ cells and gamma interferon in resolution of established Cryptosporidium parvum infection in mice.
【24h】

Requirements for CD4+ cells and gamma interferon in resolution of established Cryptosporidium parvum infection in mice.

机译：CD4 +细胞和γ干扰素对已建立的小隐隐孢子虫感染小鼠的分辨能力的要求。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The importance of CD4+ cells and gamma interferon (IFN-gamma) in the resolution of established Cryptosporidium parvum infection was investigated with a murine model of cryptosporidiosis in severe combined immunodeficient (SCID) mice. C. parvum-infected SCID mice were reconstituted with spleen cells from immunocompetent donors. The recipients were able to resolve their C. parvum infection by 17 days postreconstitution. Treatment of reconstituted SCID mice with either anti-CD4 monoclonal antibodies to deplete them of CD4+ cells or with anti-IFN-gamma to neutralize IFN-gamma activity reduced or eliminated their ability to resolve C. parvum infection whereas treatment with either anti-CD8 monoclonal antibodies or anti-asialo-GM1 antibodies had no effect. We also found C. parvum-specific antibodies in serum samples from two of four reconstituted SCID mice killed on postreconstitution day 17 but not in unreconstituted SCID mice. Moreover, anti-CD4-treated mice had no detectable specific antibodies to C. parvum, whereas all mice treated with either anti-CD8 or anti-asialo-GM1 had substantial levels of specific antibodies in their serum. Although the role of the specific antibody is not known, these findings clearly indicate that resolution of an established C. parvum infection in immunologically reconstituted SCID mice is dependent on both CD4+ cells and IFN-gamma.
机译：CD4 +细胞和γ干扰素（IFN-γ）在已建立的小隐隐孢子虫感染的解决中的重要性已用严重合并免疫缺陷（SCID）小鼠的隐孢子虫病小鼠模型进行了研究。用来自具有免疫能力的供体的脾细胞重建感染小小衣原体的SCID小鼠。接受者在重建后17天就可以解决其小隐孢子虫感染。用抗CD4单克隆抗体消耗CD4 +细胞或用抗IFN-γ中和IFN-γ活性治疗重建的SCID小鼠会降低或消除其解决小球藻感染的能力，而用抗CD8单克隆抗体治疗抗体或抗-asialo-GM1抗体无效。我们还从重构后第17天杀死的四只重构SCID小鼠中的两只血清样品中发现了C. parvum特异性抗体，但在未重构的SCID小鼠中未发现。而且，用抗CD4处理的小鼠没有检测到抗小球藻的特异性抗体，而所有用抗CD8或抗亚洲人GM1处理的小鼠的血清中都有相当水平的特异性抗体。尽管尚不清楚特异性抗体的作用，但这些发现清楚地表明，在免疫重建的SCID小鼠中已确定的小隐杆线虫感染的解决方法既依赖于CD4 +细胞，又依赖于IFN-γ。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号