...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Infection and immunity >Cytokine gene expression in human peripheral blood mononuclear cells stimulated by mannoprotein constituents from Candida albicans.
【24h】

Cytokine gene expression in human peripheral blood mononuclear cells stimulated by mannoprotein constituents from Candida albicans.

机译：来自白色念珠菌的甘露糖蛋白成分刺激的人外周血单个核细胞中的细胞因子基因表达。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The expression of cytokine genes in cultures of human peripheral blood mononuclear cells (PBMC) stimulated with mannoprotein constituents (MP) of Candida albicans has been studied by means of S1 nuclease mapping analysis, polymerase chain reaction, and enzyme-linked immunosorbent assay. MP induced early, consistent, and long-lasting production of interleukin-1 beta (IL-1 beta), tumor necrosis factor alpha, and IL-6 mRNAs. Similar results were obtained when the same PBMC cultures were stimulated with the purified protein derivative (PPD) from Mycobacterium tuberculosis or with IL-2, although lower levels of IL-6 mRNA were detected in IL-2-stimulated cells than in MP- or PPD-stimulated cells. MP, PPD, and IL-2 induced appreciable levels of granulocyte-macrophage colony-stimulating factor and gamma interferon, but only MP and PPD were able to induce IL-2 mRNA. MP were unable to stimulate a consistent expression of the genes encoding for IL-4, IL-5, and IL-10, while low, sometimes barely detectable levels of these cytokine mRNAs were observed in PPD- or IL-2-stimulated PBMC cultures. When protein synthesis of MP-stimulated PBMC was inhibited by cycloheximide, a superinduction of mRNAs for IL-4 and IL-10 and, more markedly, gamma interferon was observed. Overall, these results highlight the powerful, selective induction of cytokine gene expression by MP constituents of C. albicans in human PBMC cultures, thus providing some functional clues to explain the efficient state of the anticandidal response in normal human subjects.
机译：通过S1核酸酶作图分析，聚合酶链反应和酶联免疫吸附试验研究了用白色念珠菌的甘露糖蛋白成分（MP）刺激的人外周血单个核细胞（PBMC）培养物中细胞因子基因的表达。 MP诱导白细胞介素1 beta（IL-1 beta），肿瘤坏死因子α和IL-6 mRNA的早期，一致和持久产生。当用来自结核分枝杆菌的纯化蛋白衍生物（PPD）或用IL-2刺激相同的PBMC培养物时，虽然在IL-2刺激的细胞中检测到的IL-6 mRNA水平低于MP-或PPD刺激的细胞。 MP，PPD和IL-2诱导了一定水平的粒细胞-巨噬细胞集落刺激因子和γ干扰素，但只有MP和PPD能够诱导IL-2 mRNA。 MP无法刺激编码IL-4，IL-5和IL-10的基因的一致表达，而在PPD或IL-2刺激的PBMC培养物中观察到这些细胞因子mRNA的水平较低，有时几乎检测不到。当环己酰亚胺抑制MP刺激的PBMC的蛋白质合成时，观察到IL-4和IL-10 mRNA的超诱导作用，更明显的是，γ干扰素被观察到。总体而言，这些结果突出了人PBMC培养物中白色念珠菌的MP成分对细胞因子基因表达的强大，选择性的诱导作用，从而提供了一些功能性线索来解释正常人受试者抗candidal反应的有效状态。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号