• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Infection and immunity >Characterization of specific binding of a human immunoglobulin M monoclonal antibody to lipopolysaccharide and its lipid A domain.
【24h】

Characterization of specific binding of a human immunoglobulin M monoclonal antibody to lipopolysaccharide and its lipid A domain.

机译：人免疫球蛋白M单克隆抗体与脂多糖及其脂质A结构域的特异性结合的特征。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The human immunoglobulin M monoclonal antibody HA-1A was first described as an antibody which bound specifically to the lipid A region of lipopolysaccharide (LPS) (N. N. H. Teng, H. S. Kaplan, J. M. Herbert, C. Moore, H. Douglas, A. Wunderlich, and A. Braude, Proc. Natl. Acad. Sci. USA 82:1790-1794, 1985) and provided significant protection when administered to patients with gram-negative bacteremia and shock (E. J. Ziegler, C. J. Fisher, Jr., C. L. Sprung, R. C. Straube, J. C. Sadoff, G. E. Foulke, C. H. Wortel, M. P. Fink, R. P. Dellinger, N. N. H. Teng, I. E. Allen, H. J. Berger, G. L. Knatterud, A. F. LoBuglio, C. R. Smith, and the HA-1A Sepsis Study Group, New Engl. J. Med. 324:429-436, 1992). Since that original report, questions have arisen in the scientific literature concerning the specificity of this antibody in LPS and/or lipid A binding. Experiments have, therefore, been carried out with a variety of assay formats to determine the capacity of this HA-1A antibody to bind to lipid A and LPS. Direct binding experiments with a sensitive enzyme-linked immunosorbent assay (ELISA) system have established that HA-1A will bind to purified lipid A from both Escherichia coli and Salmonella spp. These results have been confirmed by using a fluid-phase antigen-antibody competitive inhibition assay with purified lipid A and an antibody-antibody competitive inhibition assay with a monoclonal antibody with known specificity for lipid A. The HA-1A monoclonal antibody has also been shown to bind to a panel of R-chemotype LPS by ELISA and, unlike many other previously reported anti-lipid A antibodies, binding of HA-1A to R-chemotype LPS and lipid A is comparable. Although binding of HA-1A to S-LPS (smooth, wild-type LPS) could not be detected by direct ELISA, competitive inhibition experiments with some preparations of S-LPS have been able to show specific HA-1A binding. Collectively, these data confirm the binding specificity of HA-1A for the lipid A component of LPS and provide evidence that this monoclonal antibody manifests a relatively uncommon profile in its capacity to bind lipid A and R-chemotype LPS as well as some preparations of S-LPS.
机译：首先将人免疫球蛋白M单克隆抗体HA-1A描述为与脂多糖（LPS）的脂质A区特异性结合的抗体（NNH Teng，HS Kaplan，JM Herbert，C.Moore，H.Douglas，A.Wunderlich，和A. Braude，美国国家科学院学报82：1790-1794，1985），并在对革兰氏阴性菌血症和休克患者给药时提供了显着的保护（EJ Ziegler，CJ Fisher，Jr.，CL Sprung， RC Straube，JC Sadoff，GE Foulke，CH Wortel，MP Fink，RP Dellinger，NNH Teng，IE Allen，HJ Berger，GL Knatterud，AF LoBuglio，CR Smith和HA-1A脓毒症研究组，新英格兰J. Med.324：429-436，1992）。自该原始报告以来，科学文献中就该抗体在LPS和/或脂质A结合中的特异性提出了疑问。因此，已经用多种测定形式进行了实验以确定该HA-1A抗体结合脂质A和LPS的能力。使用敏感的酶联免疫吸附测定（ELISA）系统进行的直接结合实验已确定HA-1A将与大肠杆菌和沙门氏菌属的纯化脂质A结合。这些结果已通过使用纯化的脂质A的液相抗原-抗体竞争抑制分析和使用对脂质A已知特异性的单克隆抗体的抗体-抗体竞争抑制分析得到了证实。还显示了HA-1A单克隆抗体与许多先前报道的抗脂质A抗体不同，通过ELISA可以与一组R型化学LPS结合，HA-1A与R型LPS和脂质A的结合具有可比性。尽管无法通过直接ELISA检测到HA-1A与S-LPS的结合（平滑，野生型LPS），但是使用某些S-LPS制剂的竞争性抑制实验已经能够显示出特异性的HA-1A结合。这些数据共同证实了HA-1A对LPS脂质A成分的结合特异性，并提供了证据表明该单克隆抗体在结合脂质A和R-化学型LPS以及一些S制剂中表现出相对罕见的特征。 -LPS。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号