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首页> 外文期刊>Infection and immunity >Protein D of Haemophilus influenzae is not a universal immunoglobulin D-binding protein.
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Protein D of Haemophilus influenzae is not a universal immunoglobulin D-binding protein.

机译：流感嗜血杆菌的蛋白D不是通用的免疫球蛋白D结合蛋白。

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Haemophilus influenzae type b and nontypeable H. influenzae have been reported to bind human immunoglobulin D (IgD). IgD myeloma sera from five patients were tested for the ability of IgD to bind to H. influenzae. Serotype b strains bound human IgD in four of the five sera tested. IgD in the fifth serum bound strongly to type b strain MinnA but poorly to other type b strains. Additionally, IgD binding was not observed when nontypeable strains were tested. The gene for protein D, the putative IgD-binding protein, was cloned from the IgD-binding H. influenzae type b strain MinnA and expressed in Escherichia coli. IgD binding to E. coli expressing protein D was not demonstrable. Recombinant protein D was purified, and antisera were generated in rabbits. Using these rabbit sera, we detected protein D in nontypeable as well as serotype b strains by Western blotting (immunoblotting). In contrast, IgD myeloma protein 4490, which was previously reported to bind to protein D by Ruan and coworkers (M. Ruan, M. Akkoyunlu, A. Grubb, and A. Forsgren, J. Immunol. 145:3379-3384), bound strongly to both type b and nontypeable H. influenzae as well as to E. coli expressing protein D. Thus, IgD binding is a general property of H. influenzae type b strains but not a general property of nontypeable strains, although both type b and nontypeable strains produce protein D. With the exception of IgD myeloma protein 4490 binding, we have no evidence for a role of protein D in IgD binding to H. influenzae.
机译：据报道，b型流感嗜血杆菌和不可分型流感嗜血杆菌可结合人免疫球蛋白D（IgD）。测试了五名患者的IgD骨髓瘤血清IgD与流感嗜血杆菌结合的能力。血清型b毒株在测试的五种血清中有四种结合了人IgD。第五个血清中的IgD与b型毒株MinnA强烈结合，但与其他b型毒株结合很弱。此外，测试不可分型菌株时未观察到IgD结合。从结合IgD的b型流感嗜血杆菌MinnA克隆了蛋白D的基因，即推定的IgD结合蛋白，并在大肠杆菌中表达。 IgD与表达蛋白D的大肠杆菌的结合是不可证明的。纯化重组蛋白D，并在兔中产生抗血清。使用这些兔血清，我们通过蛋白质印迹（免疫印迹）检测到了不可分型和血清型B株中的蛋白D。相比之下，IgD骨髓瘤蛋白4490（以前曾被Ruan和同事报道与蛋白D结合）（M。Ruan，M。Akkoyunlu，A。Grubb和A. Forsgren，J. Immunol。145：3379-3384）， IgD结合是b型和不可分型流感嗜血杆菌与表达蛋白D的大肠杆菌的强力结合。因此，IgD结合是b型流感嗜血菌菌株的一般特性，但不是b型流感嗜血菌的一般特性除IgD骨髓瘤蛋白4490结合外，我们没有证据表明蛋白D在IgD与流感嗜血杆菌的结合中的作用。

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