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首页> 外文期刊>Infection and immunity >Roles of CR3 and mannose receptors in the attachment and ingestion of Leishmania donovani by human mononuclear phagocytes.
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Roles of CR3 and mannose receptors in the attachment and ingestion of Leishmania donovani by human mononuclear phagocytes.

机译：CR3和甘露糖受体在人单核吞噬细胞附着和摄取利什曼原虫中的作用。

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Leishmania donovani is an obligate intracellular parasite of mammalian macrophages. Two macrophage receptors, the mannose-fucose receptor (MFR) and the receptor for complement component C3bi, CR3, were examined for their roles in the attachment and ingestion of L. donovani by human monocyte-derived macrophages. Two monoclonal antibodies which bind to the human CR3, anti-Mo1 and anti-Mac-1, inhibited both attachment and ingestion of L. donovani promastigotes after preincubation with human monocyte-derived macrophages; attachment was inhibited by 40 and 62% by anti-Mo1 and anti-Mac-1, respectively, and ingestion was inhibited by 34 and 51% by anti-Mo1 and anti-Mac-1, respectively. The interaction between promastigotes and CR3 may not have involved the C3bi-binding site on CR3, however, because a monoclonal antibody which exhibits specificity for this site, OKM10, inhibited promastigote attachment by only 18%. In contrast, OKM1, which is believed to react with the alternate lectinlike binding site on CR3, inhibited ingestion by 65%. MFR activity was inhibited using the soluble MFR ligands, mannan and mannosylated bovine serum albumin, which also inhibited promastigote attachment by 40 and 37%, respectively. The simultaneous inhibition of both CR3 (by anti-Mac-1) and the MFR (by either mannan or mannosylated bovine serum albumin) resulted in a greater decrease in promastigote attachment than inhibition of either receptor alone. Additionally, the reduction of MFR activity by allowing macrophages to adhere to a mannan-coated surface followed by the addition of anti-CR3 antibodies resulted in an 81% inhibition of promastigote ingestion, a greater decrease than was obtained by manipulation of either receptor alone. The results suggest that the MFR and CR3 independently participate in the attachment and ingestion of L. donovani promastigotes by human macrophages.
机译：Leishmania donovani是哺乳动物巨噬细胞的专性细胞内寄生虫。检查了两个巨噬细胞受体，甘露糖-岩藻糖受体（MFR）和补体成分C3bi受体CR3在人类单核细胞衍生巨噬细胞对L. donovani的附着和摄取中的作用。在与人单核细胞衍生的巨噬细胞预温育后，两种与人CR3结合的单克隆抗体抗Mo1和抗Mac-1抑制了多诺氏乳杆菌前体的附着和摄取。抗Mo1和抗Mac-1分别抑制40％和62％的附着，抗Mo1和抗Mac-1分别抑制34和51％的摄取。前鞭毛体与CR3之间的相互作用可能不涉及CR3上的C3bi结合位点，因为对这种位点具有特异性的单克隆抗体OKM10仅将前体弓形体的附着抑制了18％。相反，据信OKM1与CR3上的其他凝集素样结合位点反应，抑制了摄食65％。使用可溶性MFR配体，甘露聚糖和甘露糖基化的牛血清白蛋白可抑制MFR活性，后者还可分别将前鞭毛体附着抑制40％和37％。与单独抑制任一受体相比，同时抑制CR3（通过抗Mac-1）和MFR（通过甘露聚糖或甘露糖基化的牛血清白蛋白）都导致了前鞭毛体附着的减少更大。另外，通过使巨噬细胞粘附于甘露聚糖包被的表面，然后添加抗CR3抗体而降低了MFR活性，从而导致对前鞭毛体摄食的抑制作用达到了81％，与单独操作任一受体所获得的抑制作用相比，下降幅度更大。结果表明，MFR和CR3独立参与人巨噬细胞对多诺氏乳杆菌前鞭毛体的附着和摄取。

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