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首页> 外文期刊>Infection and immunity >Aerobactin and other virulence factor genes among strains of Escherichia coli causing urosepsis: association with patient characteristics.
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Aerobactin and other virulence factor genes among strains of Escherichia coli causing urosepsis: association with patient characteristics.

机译：引起尿毒症的大肠杆菌菌株中的气杆菌素和其他毒力因子基因：与患者特征有关。

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To assess the role of aerobactin as a virulence factor among uropathogenic Escherichia coli, we determined the prevalence, location, and phenotypic expression of aerobactin determinants among 58 E. coli strains causing bacteremic urinary tract infections. We correlated the presence of the aerobactin system with antimicrobial-agent resistance, the presence and phenotypic expression of other uropathogenic virulence factor determinants (P fimbriae, hemolysin, and type 1 fimbriae), and characteristics of patients. Colony and Southern hybridization of total and plasmid DNA with DNA probes for each virulence factor showed that aerobactin determinants were present in 78% of the strains and were plasmid associated in 21%, whereas P fimbria, hemolysin, and type 1 fimbria determinants were present in 74, 43, and 98% of the strains, respectively, and were always chromosomal. Chromosomal aerobactin, P fimbria, and hemolysin determinants occurred together on the chromosome more often in strains from patients without predisposing urological or medical conditions (P = 0.04). Strains with plasmid-encoded aerobactin lacked determinants for P fimbriae (P = 0.004) and hemolysin (P = 0.0004), were resistant to multiple antimicrobial agents (P = 0.0001), and were found only in compromised patients. Mating experiments demonstrated that some aerobactin plasmids also encoded antimicrobial-agent resistance. These findings suggest that the determinants for aerobactin, P fimbriae, and hemolysin are conserved on the chromosome of the antimicrobial-agent-susceptible uropathogenic strains of E. coli which invade noncompromised patients. In contrast, these chromosomal virulence factors are often absent from E. coli strains causing urosepsis in compromised hosts; these strains may acquire plasmid aerobactin in conjunction with antimicrobial-agent resistance genes.
机译：为了评估航空细菌素作为尿毒症致病性大肠杆菌中的致病因子的作用，我们确定了引起细菌性尿路感染的58株大肠杆菌中航空细菌素决定簇的流行，位置和表型表达。我们将航空细菌素系统的存在与抗微生物剂耐药性，其他尿路致病性毒力决定因素（P菌毛，溶血素和1型菌毛）的存在和表型表达以及患者的特征相关联。对每种毒力因子的总DNA和质粒DNA进行DNA探针的菌落和Southern杂交显示，在78％的菌株中存在航空杆菌素决定簇，在21％的菌株中与质粒相关，而在P菌毛，溶血素和1型菌毛决定簇中分别有74、43和98％的菌株是染色体的。在没有易患泌尿科或医学疾病的情况下，来自患者的菌株中染色体的气杆菌素，P菌毛和溶血素决定簇在染色体上的发生频率更高（P = 0.04）。带有质粒编码的气杆菌素的菌株缺乏P菌毛（P = 0.004）和溶血素（P = 0.0004）的决定因素，对多种抗菌药物具有耐药性（P = 0.0001），仅在受损患者中发现。交配实验表明，某些航空细菌素质粒也编码抗微生物剂。这些发现表明，气旋素，P菌毛和溶血素的决定因素在侵染非感染性患者的大肠杆菌的抗微生物药物敏感性尿路致病菌株的染色体上是保守的。相反，在致病宿主中引起尿毒症的大肠杆菌菌株通常不存在这些染色体致病因子。这些菌株可与抗微生物剂耐药基因一起获得质粒气杆菌素。

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