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首页> 外文期刊>Infection and immunity >Amastigote stage-specific monoclonal antibodies against Leishmania major.
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Amastigote stage-specific monoclonal antibodies against Leishmania major.

机译：抗主要利什曼原虫的鞭毛虫阶段特异性单克隆抗体。

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Monoclonal antibodies were produced against gamma-irradiated amastigotes of Leishmania major. Five antibodies (T16 through T20) were selected which reacted in enzyme-linked immunoassays with the intracellular stage of the parasite. These antibodies did not react with promastigotes of L. major or Leishmania donovani. One of the monoclonal antibodies (T16) reacted with amastigotes of Leishmania mexicana amazonensis and L. donovani. Western blotting (immunoblotting) and immunoprecipitation of [35S]methionine-labeled amastigotes demonstrates that T16 reacted with multiple L. major amastigote components between 12 and 180 kilodaltons. Antibody T20 was shown to recognize a low-molecular-mass doublet (less than 26 kilodaltons) in both [14C]leucine- and [35S]methionine-labeled amastigotes. A protein of less than 180 kilodaltons was also weakly recognized by T17, T19, and T20 in metabolically labeled amastigotes. This protein reacted strongly with T16. The reactive antigens could be identified on the surface of amastigotes isolated from the lesions of infected mice and on newly transformed amastigotes within 24 h after the infection of mouse peritoneal macrophages by promastigotes. These monoclonal antibodies should prove useful for the diagnosis of L. major in human tissue biopsies.
机译：产生了针对大剂量利什曼原虫经γ射线辐照的变形虫的单克隆抗体。选择了五种抗体（T16至T20），它们在酶联免疫分析中与寄生虫的细胞内阶段反应。这些抗体不与L.major或Leishmania donovani的前鞭毛体反应。一种单克隆抗体（T16）与墨西哥利什曼原虫和杜氏利什曼原虫的变形虫反应。 Western印迹（免疫印迹）和[35S]蛋氨酸标记的amastigotes的免疫沉淀表明，T16与12至180道尔顿之间的多个乳杆菌主要鞭毛体成分反应。已显示抗体T20在[14C]亮氨酸和[35S]蛋氨酸标记的amastigotes中都能识别低分子双峰态（小于26道尔顿）。 T17，T19和T20在代谢标记的amastigotes中也弱识别不到180千道尔顿的蛋白质。该蛋白质与T16强烈反应。在前鞭毛体感染小鼠腹膜巨噬细胞后的24小时内，可以在从感染小鼠的病变中分离出的变形虫表面以及新转化的变形虫中鉴定出反应性抗原。这些单克隆抗体应被证明可用于诊断人体组织活检中的大链球菌。

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