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首页> 外文期刊>Infection and immunity >Partial biochemical characterization of cell surface hydrophobicity and hydrophilicity of Candida albicans.
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Partial biochemical characterization of cell surface hydrophobicity and hydrophilicity of Candida albicans.

机译：白色念珠菌细胞表面疏水性和亲水性的部分生化特征。

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Hydrophobic yeast cells of Candida albicans are more virulent than hydrophilic yeast cells in mice. Results of experiments performed in vitro suggest that surface hydrophobicity contributes to virulence in multiple ways. Before definitive studies in vivo concerning the contribution of fungal surface hydrophobicity to pathogenesis can be performed, biochemical, physiological, and immunochemical characterization of the macromolecules responsible for surface hydrophobicity must be accomplished. This report describes our initial progress toward this goal. When hydrophobic and hydrophilic yeast cells of C. albicans were exposed to various enzymes, only proteases caused any change in surface hydrophobicity. Hydrophobic cell surfaces were sensitive to trypsin, chymotrypsin, pronase E, and pepsin. This indicates that surface hydrophobicity is due to protein. Papain, however, had no significant effect. The hydrophobicity of hydrophilic cells was altered only by papain. The proteins responsible for surface hydrophobicity could be removed by exposure to lyticase, a beta 1-3 glucanase, for 30 to 60 min. When 60-min lyticase digests of hydrophobic and hydrophilic cell walls were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) with a 12.5% resolving gel, each protein population contained a single unique protein that was not evident in the other protein population. However, when the cell wall surface proteins of hydrophobic and hydrophilic cells were first labeled with 125I and then removed by lyticase and analyzed by SDS-PAGE, at least four low-molecular-mass (less than 65 kilodaltons) proteins associated with hydrophobic cells were either absent or much less abundant in the hydrophilic cell digests. This result was seen for both C. albicans strains that we tested. When late-exponential-phase hydrophilic cells were treated with tunicamycin, high levels of surface hydrophobicity were obtained by stationary phase. These results indicate that the surface hydrophobicity of C. albicans reflects changes in external surface protein exposure and that protein mannosylation may influence exposure of hydrophobic surface proteins.
机译：在小鼠中，白色念珠菌的疏水性酵母细胞比亲水性酵母细胞更具毒性。体外实验的结果表明，表面疏水性以多种方式促进了毒性。在可以进行有关真菌表面疏水性对致病作用的体内确定性研究之前，必须完成负责表面疏水性的大分子的生物化学，生理和免疫化学表征。本报告描述了我们朝着这一目标的初步进展。当白色念珠菌的疏水和亲水酵母细胞暴露于各种酶时，只有蛋白酶引起表面疏水性的任何变化。疏水性细胞表面对胰蛋白酶，胰凝乳蛋白酶，链霉蛋白酶E和胃蛋白酶敏感。这表明表面疏水性归因于蛋白质。木瓜蛋白酶没有明显的作用。亲水细胞的疏水性仅被木瓜蛋白酶改变。可通过暴露于裂解酶（β1-3葡聚糖酶）30至60分钟来去除负责表面疏水性的蛋白质。通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）和12.5％分离凝胶分析60分钟的疏水性和亲水性细胞壁裂解酶消化液时，每个蛋白质群体均包含一个独特的蛋白质，而其他蛋白质中没有人口。但是，当疏水性和亲水性细胞的细胞壁表面蛋白首先用125 I标记，然后通过裂解酶去除并通过SDS-PAGE分析时，至少有四个与疏水性细胞相关的低分子质量（小于65道尔顿）的蛋白被分离出来。在亲水性细胞消化液中不存在或不大量存在。对于我们测试的两个白色念珠菌菌株都可以看到该结果。当用衣霉素处理晚期指数期亲水细胞时，通过固定相获得高水平的表面疏水性。这些结果表明白色念珠菌的表面疏水性反映了外表面蛋白暴露的变化，并且蛋白质甘露糖基化作用可能会影响疏水表面蛋白的暴露。

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