• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Infection and immunity >Hemagglutinin B Is Involved in the Adherence of Porphyromonas gingivalis to Human Coronary Artery Endothelial Cells
【24h】

Hemagglutinin B Is Involved in the Adherence of Porphyromonas gingivalis to Human Coronary Artery Endothelial Cells

机译：血凝素B参与牙龈卟啉单胞菌对人冠状动脉内皮细胞的粘附

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Porphyromonas gingivalis is a periodontopathogen that may play a role in cardiovascular diseases. Hemagglutinins may function as adhesins and are required for virulence of several bacterial pathogens. The aim of this study was to determine the role of hemagglutinin B (HagB) in adherence of P. gingivalis to human coronary artery endothelial (HCAE) cells. P. gingivalis strain 381, a P. gingivalis 381 HagB mutant, Escherichia coli JM109 expressing HagB (E. coli-HagB), and E. coli JM109 containing pUC9 (E. coli-pUC9) were tested for their ability to attach to HCAE cells. Inhibition assays were performed to determine the ability of purified recombinant HagB (rHagB) as well as antibodies to HagB, including the polyclonal antibody (PAb) A7985 and the monoclonal antibody (MAb) HL1858, to inhibit the attachment of P. gingivalis to HCAE cells. As expected, when the attachment of P. gingivalis and the HagB mutant were compared, no statistical significance was observed between the two groups (P = 0.331), likely due to the expression of the hagB homolog hagC. However, E. coli-HagB adhered significantly better to HCAE cells than did E. coli-pUC9, the control strain. In a competition assay, the presence of purified rHagB decreased bacterial adhesion of P. gingivalis or E. coli-HagB to HCAE cells. The presence of PAb A7985 or MAb HL1858 also significantly decreased attachment of P. gingivalis and E. coli-HagB to host cells. These results indicate that HagB is involved in the adherence of P. gingivalis to human primary endothelial cells.
机译：牙龈卟啉单胞菌是一种牙周病原体，可能在心血管疾病中起作用。血凝素可作为粘附素起作用，并且是几种细菌病原体的毒性所必需的。这项研究的目的是确定血凝素B（HagB）在 P依从性中的作用。牙龈对人冠状动脉内皮细胞（HCAE）的影响。 P。牙龈菌菌株381， P。 gingivalis 381 HagB突变体，表达HagB的大肠杆菌 JM109（大肠杆菌 -HagB）和 E。测试了含有pUC9（大肠杆菌 -pUC9）的大肠杆菌 JM109附着于HCAE细胞的能力。进行抑制测定以确定纯化的重组HagB（rHagB）以及针对HagB的抗体（包括多克隆抗体（PAb）A7985和单克隆抗体（MAb）HL1858）抑制 P附着的能力。牙龈炎进入HCAE细胞。不出所料，当附加 P时。比较了牙龈炎和HagB突变体，两组之间均未发现统计学意义（ P = 0.331），这可能是由于 hagB 同源物的表达 hagC 。但是， E。 coli- HagB与HCAE细胞的粘附性明显优于 E。大肠杆菌pUC9，对照菌株。在竞争试验中，纯化的rHagB的存在降低了 P的细菌粘附。 gingivalis 或 E。大肠杆菌-HagB转HCAE细胞。 PAb A7985或MAb HL1858的存在也显着降低了 P的附着。 gingivalis 和 E。大肠杆菌-HagB感染宿主细胞。这些结果表明HagB参与了 P的遵守。牙龈对人原代内皮细胞的影响

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号