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首页> 外文期刊>Infection and immunity >Variations in Helicobacter pylori Lipopolysaccharide To Evade the Innate Immune Component Surfactant Protein D
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Variations in Helicobacter pylori Lipopolysaccharide To Evade the Innate Immune Component Surfactant Protein D

机译：幽门螺杆菌脂多糖的变异，以逃避先天免疫成分表面活性剂蛋白D

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摘要

Helicobacter pylori is a common and persistent human pathogen of the gastric mucosa. Surfactant protein D (SP-D), a component of innate immunity, is expressed in the human gastric mucosa and is capable of aggregating H. pylori. Wide variation in the SP-D binding affinity to H. pylori has been observed in clinical isolates and laboratory-adapted strains. The aim of this study was to reveal potential mechanisms responsible for evading SP-D binding and establishing persistent infection. An escape variant, J178V, was generated in vitro, and the lipopolysaccharide (LPS) structure of the variant was compared to that of the parental strain, J178. The genetic basis for structural variation was explored by sequencing LPS biosynthesis genes. SP-D binding to clinical isolates was demonstrated by fluorescence-activated cell sorter analyses. Here, we show that H. pylori evades SP-D binding through phase variation in lipopolysaccharide. This phenomenon is linked to changes in the fucosylation of the O chain, which was concomitant with slipped-strand mispairing in a poly(C) tract of the fucosyltransferase A (fucT1) gene. SP-D binding organisms are predominant in mucus in vivo (P = 0.02), suggesting that SP-D facilitates physical elimination. Phase variation to evade SP-D contributes to the persistence of this common gastric pathogen.
机译：幽门螺杆菌是胃粘膜常见且持久的人类病原体。表面活性蛋白D（SP-D）是先天免疫的组成部分，在人胃粘膜中表达，能够聚集H。幽门炎。 SP-D与 H的结合亲和力有很大差异。在临床分离株和实验室适应菌株中观察到幽门螺旋杆菌。这项研究的目的是揭示负责逃避SP-D绑定和建立持久性感染的潜在机制。体外产生了逃逸变体J178V，并将该变体的脂多糖（LPS）结构与亲本菌株J178的结构进行了比较。通过对LPS生物合成基因进行测序，探索了结构变异的遗传基础。 SP-D与临床分离株的结合已通过荧光激活的细胞分选分析得到证实。在这里，我们显示 H。幽门螺杆菌通过脂多糖的相变逃避SP-D结合。这种现象与O链岩藻糖基化的变化有关，这与岩藻糖基转移酶A（ fucT1 ）基因的多聚C链中的滑链错配有关。 SP-D结合生物是体内粘液中的主要成分（ P = 0.02），这表明SP-D有助于物理消除。规避SP-D的相变有助于这种常见胃病原体的持久性。

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