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首页> 外文期刊>Infection and immunity >Regulation of a Type III and a Putative Secretion System in Edwardsiella tarda by EsrC Is under the Control of a Two-Component System, EsrA-EsrB
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Regulation of a Type III and a Putative Secretion System in Edwardsiella tarda by EsrC Is under the Control of a Two-Component System, EsrA-EsrB

机译：EsrC调节爱德华氏菌在爱德华氏菌中的III型和推测的分泌系统受两组分系统EsrA-EsrB的控制

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Edwardsiella tarda is a gram-negative enteric pathogen that causes hemorrhagic septicemia in fish and gastro- and extraintestinal infections in humans. A type III secretion system (TTSS) and a putative secretion system (EVP) have been found to play important roles in E. tarda pathogenesis. Our previous studies suggested that the TTSS and EVP gene clusters were regulated by a two-component system of EsrA-EsrB. In the present study, we characterized another regulator, EsrC, which showed significant sequence similarity to the AraC family of transcriptional regulators. Mutants with in-frame deletions of esrC increased the 50% lethal doses in blue gourami fish, reduced extracellular protein production, and failed to aggregate. Complementation of esrC restored these three phenotypes. Two-dimensional gel electrophoresis showed that EsrC regulated the expression of secreted proteins encoded by the TTSS (such as EseB and EseD) and EVP (EvpC) gene clusters. The expression of esrC required a functional two-component system of EsrA-EsrB. EsrC in turn regulated the expression of selected genes encoded in TTSS (such as the transcriptional unit of orf29and orf30, but not esaC) and genes encoded in the EVP gene cluster. The present study sheds light on the regulation of these two key virulence-associated secretion systems and provides greater insight into the pathogenic mechanisms of this bacterium.
机译： Edwardsiella tarda 是一种革兰氏阴性肠道病原体，可导致鱼类鱼类败血性败血症和人类胃肠道感染。已经发现III型分泌系统（TTSS）和推定的分泌系统（EVP）在E中起着重要作用。 tarda 的发病机理。我们以前的研究表明，TTSS和EVP基因簇受EsrA-EsrB两组分系统调控。在本研究中，我们表征了另一种调节子EsrC，它与转录调节子AraC家族表现出显着的序列相似性。具有 esrC 框内缺失的突变体在蓝色古拉米鱼中增加了50％的致死剂量，减少了细胞外蛋白质的产生，并且无法聚集。 esrC 的互补可恢复这三种表型。二维凝胶电泳显示，EsrC调节由TTSS（例如EseB和EseD）和EVP（EvpC）基因簇编码的分泌蛋白的表达。 esrC 的表达需要EsrA-EsrB的功能性两组分系统。 EsrC反过来调节TTSS中编码的选定基因（例如 orf29 和 orf30 的转录单位，但不是 esaC 的转录单位）的表达编码在EVP基因簇中。本研究阐明了这两个与毒力相关的关键分泌系统的调控，并提供了对该细菌的致病机制的更深入的了解。

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