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Mouse Model of Enteropathogenic Escherichia coli Infection

机译：肠致病性大肠杆菌感染的小鼠模型

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Enteropathogenic Escherichia coli (EPEC) is an important cause of diarrhea in humans. EPEC infection of cultured intestinal epithelial cells induces attaching and effacing (A/E) lesions, alters intestinal ion transport, increases paracellular permeability, and stimulates inflammation. The lack of a small-animal model has restricted in vivo studies examining EPEC-host interactions. The aim of this study was to characterize the C57BL/6J mouse as a model of EPEC infection. We have shown that EPEC can adhere to and colonize the intestinal epithelium of C57BL/6J mice. Animal weight and water intake were not altered during 10 days of EPEC infection. The proximal colon of infected mice contained semisolid stool, with stool pellets forming only in the distal colon. In contrast, the entire colon of control mice contained formed stool. Microvillous effacement and actin rearrangement, characteristic of A/E lesions, were seen in the intestine of infected mice but not control mice. Histological assessment revealed increased numbers of lamina propria neutrophils with occasional crypt abscesses, intraepithelial lymphocytes, and goblet cells in the intestine of EPEC-infected mice. Altogether, these data suggest that the C57BL/6J mouse is susceptible to infection by EPEC and will provide a suitable in vivo model for studying the consequences of EPEC infection.
机译：肠致病性大肠埃希菌（EPEC）是人类腹泻的重要原因。培养的肠上皮细胞的EPEC感染会引起附着和脱落（A / E）损伤，改变肠离子运输，增加细胞旁通透性并刺激炎症。缺少小动物模型限制了体内研究EPEC-宿主相互作用的研究。这项研究的目的是将C57BL / 6J小鼠表征为EPEC感染的模型。我们已经表明，EPEC可以粘附并定植在C57BL / 6J小鼠的肠上皮中。在EPEC感染的10天内，动物的体重和摄水量没有改变。感染小鼠的近端结肠含有半固体粪便，粪便颗粒仅在远端结肠形成。相反，对照小鼠的整个结肠含有形成的粪便。在受感染小鼠的肠道中可见到微毛绒毛和肌动蛋白重排，这是A / E病变的特征，但未见对照小鼠。组织学评估显示，在EPEC感染的小鼠肠中，固有层中性粒细胞数量增加，偶有隐窝脓肿，上皮内淋巴细胞和杯状细胞。总而言之，这些数据表明C57BL / 6J小鼠易受EPEC感染，并将为研究EPEC感染的后果提供合适的体内模型。

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