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首页> 外文期刊>Infection and immunity >Responses of Well-Differentiated Airway Epithelial Cell Cultures from Healthy Donors and Patients with Cystic Fibrosis to Burkholderia cenocepacia Infection
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Responses of Well-Differentiated Airway Epithelial Cell Cultures from Healthy Donors and Patients with Cystic Fibrosis to Burkholderia cenocepacia Infection

机译：健康捐献者和囊性纤维化患者分化良好的气道上皮细胞培养对伯克霍尔德菌感染的反应

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Well-differentiated cultures established from airway epithelia of patients with cystic fibrosis (CF cultures) exhibited goblet cell hyperplasia, increased secretion of mucus, and higher basal levels of interleukin-8 than similarly cultured cells from healthy donors. Upon apical infection with low doses (104 to 105 CFU) of Burkholderia cenocepacia isolate BC7, the two cultures gave different responses. While normal cultures trapped the added bacteria in the mucus layer, killed and/or inhibited bacterial replication, and prevented bacterial invasion of the cells, CF cultures failed to kill and/or supported the growth of bacteria, leading to invasion of underlying epithelial cells, compromised transepithelial permeability, and cell damage. Depletion of the surface mucus layer prior to bacterial infection rendered the normal cultures susceptible to bacterial invasion, but the invading bacteria were mainly confined to vacuoles within the cells and appeared to be nonviable. In contrast, bacteria that invaded cells in CF cultures were found free in the cytoplasm surrounded by intermediate filaments and also between cells. Cultured CF airway epithelium was therefore more susceptible to infection than normal epithelium. This mimics CF tissue in vivo and illustrates differences in the way epithelia in CF patients and normal subjects handle bacterial infection. In addition, we found that the CF and normal cell cultures responded differently not only to isolate BC7 but also to isolates of other B. cepacia complex species. We therefore conclude that this cell culture model is suitable for investigation of B. cepacia complex pathogenesis in CF patients.
机译：从囊性纤维化患者的气道上皮建立的高分化培养物（CF培养物）与健康供体的类似培养细胞相比，显示出杯状细胞增生，粘液分泌增加和白细胞介素8的基础水平更高。小剂量伯克霍尔德森菌分离株BC7的低剂量（10 4 至10 5 CFU）根尖感染后，两种培养物的反应不同。正常培养物会将添加的细菌捕获在粘液层中，杀死和/或抑制细菌复制，并阻止细菌入侵细胞，而CF培养物却无法杀死和/或支持细菌生长，从而导致潜在的上皮细胞入侵，受损的上皮通透性和细胞损伤。细菌感染前表面粘液层的耗尽使正常培养物容易受到细菌侵袭，但入侵的细菌主要局限于细胞内的液泡中，似乎是不可行的。相反，在CF培养基中侵入细胞的细菌在被中间细丝包围的细胞质中以及细胞之间都没有发现。因此，培养的CF气道上皮比正常上皮更容易感染。这在体内模拟了CF组织，并说明了CF患者和正常受试者处理细菌感染的上皮细胞方式的差异。此外，我们发现CF和正常细胞培养物不仅对BC7分离株反应不同，而且对其他 B分离株的反应也不同。 cepacia 复杂物种。因此，我们得出结论，该细胞培养模型适合于 B的研究。 CF患者的cepacia 复杂发病机制。

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