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A Type III Secretion System Is Required for Aeromonas hydrophila AH-1 Pathogenesis

机译：嗜水气单胞菌AH-1发病机理需要III型分泌系统

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Aeromonas hydrophila is a gram-negative opportunistic pathogen in fish and humans. Many bacterial pathogens of animals and plants have been shown to inject anti-host virulence determinants into the hosts via a type III secretion system (TTSS). Degenerate primers based on lcrD family genes that are present in every known TTSS allowed us to locate the TTSS gene cluster in A. hydrophila AH-1. A series of genome walking steps helped in the identification of 25 open reading frames that encode proteins homologous to those in TTSSs in other bacteria. PCR-based analysis showed the presence of lcrD homologs (ascV) in all of the 33 strains of A. hydrophila isolated from various sources. Insertional inactivation of two of the TTSS genes (aopB and aopD) led to decreased cytotoxicity in carp epithelial cells, increased phagocytosis, and reduced virulence in blue gourami. These results show that a TTSS is required for A. hydrophila pathogenesis. This is the first report of sequencing and characterization of TTSS gene clusters from A. hydrophila. The TTSS identified here may help in developing suitable vaccines as well as in further understanding of the pathogenesis of A. hydrophila.
机译：嗜水气单胞菌是鱼类和人类中革兰氏阴性机会病原体。已经显示出许多动植物的细菌病原体可通过III型分泌系统（TTSS）将抗宿主毒力决定簇注入宿主。基于每个已知TTSS中存在的 lcrD 家族基因的简并引物使我们能够将TTSS基因簇定位在 A中。亲水性 AH-1。一系列的基因组行走步骤有助于鉴定25个开放阅读框，这些阅读框编码的蛋白质与其他细菌中TTSS中的蛋白质同源。基于PCR的分析表明，在所有33种 A菌株中均存在 lcrD 同源物（ ascV ）。从各种来源分离出的亲水性。 TTSS中的两个基因（ aopB 和 aopD ）的插入失活导致鲤鱼上皮细胞的细胞毒性降低，吞噬作用增强和蓝钩吻鱼的致病力降低。这些结果表明 A需要TTSS。亲水性发病机理。这是来自 A的TTSS基因簇的测序和表征的首次报道。亲水。此处确定的TTSS可能有助于开发合适的疫苗以及进一步了解 A的发病机理。亲水。

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