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首页> 外文期刊>Infection and immunity >Induction of Morphological and Electrophysiological Changes in Hamster Cornea after In Vitro Interaction with Trophozoites of Acanthamoeba spp.
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Induction of Morphological and Electrophysiological Changes in Hamster Cornea after In Vitro Interaction with Trophozoites of Acanthamoeba spp.

机译：与棘轮藻属滋养体体外相互作用后仓鼠角膜的形态和电生理变化的诱导。

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摘要

Acanthamoeba castellani and Acanthamoeba polyphaga are free-living amebae that cause keratitis and granulomatous encephalitis in humans. We have analyzed the early morphological and electrophysiological changes occurring during the in vitro interaction of cultured amebae with intact or physically damaged corneas obtained from hamsters. Both species of Acanthamoeba produced similar cytopathic changes, as seen by light microscopy and scanning electron microscopy. After adhesion to the epithelial surface, trophozoites formed clumps and migrated toward the cell borders, causing the separation of adjacent cells at 1 h of coculture. At later stages (2 to 4 h), some amebae were found under desquamating epithelial cells whereas others were seen associated with damaged cells or forming amebostome-like structures to ingest detached epithelial cells. Control corneas incubated in culture medium conditioned with amebae showed a cytoplasmic vacuolization and blurring of the epithelial-stromal junction. The early stages of corneal epithelial damage caused by amebae were also analyzed by measuring the transepithelial resistance changes in corneas mounted in Ussing chambers. Both species of Acanthamoeba caused a rapid decrease in electrical resistance. The present observations demonstrate that under in vitro conditions, Acanthamoeba trophozoites rapidly cause significant damage to the corneal epithelium. Furthermore, in our experimental model, previous physical damage to the corneas was not a prerequisite for the development of amebic corneal ulcerations.
机译： Acanthamoeba castellani 和 Acanthamoeba polyphaga 是可导致人角膜炎和肉芽肿性脑炎的自由生存的变形虫。我们分析了培养的变形虫与从仓鼠获得的完整或物理受损的角膜在体外相互作用期间发生的早期形态和电生理变化。如光学显微镜和扫描电子显微镜所见，两种棘阿米巴细菌都产生相似的细胞病变。粘附到上皮表面后，滋养体形成团块并向细胞边界迁移，在共培养1 h时导致相邻细胞分离。在随后的阶段（2至4小时），在浸润的上皮细胞下发现了一些变形虫，而另一些则与受损的细胞相关或形成了变形虫样结构，以摄取脱落的上皮细胞。在用阿米巴调节的培养基中孵育的对照角膜显示出细胞质空泡化和上皮-基质连接的模糊。还通过测量安装在Usssing腔室中角膜的经上皮阻力变化来分析由amebae引起的角膜上皮损伤的早期阶段。棘阿米巴这两个物种都导致电阻迅速降低。本观察结果表明，在体外条件下， Acanthamoeba 滋养体迅速引起对角膜上皮的明显损害。此外，在我们的实验模型中，先前对角膜的物理损伤不是发展阿米巴角膜溃疡的先决条件。

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