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Inactivation of Streptococcus gordonii SspAB Alters Expression of Multiple Adhesin Genes

机译：戈登链球菌SspAB的失活改变了多个粘附素基因的表达。

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SspA and SspB (antigen I/II family proteins) can bind Streptococcus gordonii to other oral bacteria and also to salivary agglutinin glycoprotein, a constituent of the salivary film or pellicle that coats the tooth. To learn if SspA and SspB are essential for adhesion and initial biofilm formation on teeth, S. gordonii DL1 was incubated with saliva-coated hydroxyapatite (sHA) for 2 h in Todd-Hewitt broth with 20% saliva to develop initial biofilms. Sessile cells attached to sHA, surrounding planktonic cells, and free-growing cells were recovered separately. Free-growing cells expressed more sspA-specific mRNA and sspB-specific mRNA than sessile cells. Free-growing cells expressed the same levels of sspA and sspB as planktonic cells. Surprisingly, an SspA? SspB? mutant strain showed 2.2-fold greater biofilm formation on sHA than wild-type S. gordonii DL1. To explain this observation, we tested the hypothesis that inactivation of sspA and sspB genes altered the expression of other adhesin genes during initial biofilm formation in vitro. When compared to wild-type cells, expression of scaA and abpB was significantly up-regulated in the SspA? SspB? strain in sessile, planktonic, and free-growing cells. Consistent with this finding, ScaA antigen was also overexpressed in planktonic and free-growing SspA? SspB? cells compared to the wild type. SspA/B adhesins, therefore, were strongly suggested to be involved in the regulation of multiple adhesin genes.
机译：SspA和SspB（I / II抗原家族蛋白）可以将链球菌 戈登氏与其他口腔细菌结合，也可以与唾液凝集素糖蛋白结合，唾液凝集素糖膜的成分牙齿。要了解SspA和SspB对于粘附和牙齿上初始生物膜形成是否必不可少， S。将戈登氏DL1与唾液包被的羟基磷灰石（sHA）在含有20％唾液的Todd-Hewitt肉汤中孵育2小时，以形成初始生物膜。分别回收了附着在sHA上的无精细胞，周围的浮游细胞和自由生长的细胞。自由生长的细胞表达的 sspA 特异性mRNA和 sspB 的mRNA高于无柄细胞。自由生长的细胞表达与浮游细胞相同水平的 sspA 和 sspB 。令人惊讶的是，SspA ？ SspB ？突变株在sHA上的生物膜形成比野生型 S高2.2倍。 gordonii DL1。为了解释这一发现，我们测试了在体外初始生物膜形成过程中 sspA 和 sspB 基因失活会改变其他粘附素基因表达的假说。与野生型细胞相比，SspA ？ SspB ？中 scaA 和 abpB 的表达明显上调。无柄，浮游和自由生长细胞中的菌株。与该发现一致，与野生型相比，ScaA抗原在浮游和自由生长的SspA ？ SspB ？细胞中也过表达。因此，强烈建议SspA / B黏附素参与多种黏附素基因的调控。

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