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首页> 外文期刊>Infection and immunity >Roles of CD4+ T Cells and Gamma Interferon in Protective Immunity against Babesia microtiInfection in Mice
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Roles of CD4+ T Cells and Gamma Interferon in Protective Immunity against Babesia microtiInfection in Mice

机译：CD4 + T细胞和γ干扰素在抵抗小鼠小儿巴贝虫感染的保护性免疫中的作用

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Babesia microti produces a self-limiting infection in mice, and recovered mice are resistant to reinfection. In the present study, the role of T cells in protective immunity against challenge infection was examined. BALB/c mice which recovered from primary infection showed strong protective immunity against challenge infection. In contrast, nude mice which failed to control the primary infection and were cured with an antibabesial drug did not show protection against challenge infection. Treatment of immune mice with anti-CD4 monoclonal antibody (MAb) diminished the protective immunity against challenge infection, but treatment with anti-CD8 MAb had no effect on the protection. Transfer of CD4+ T-cell-depleted spleen cells resulted in higher parasitemia than transfer of CD8+ T-cell-depleted spleen cells. A high level of gamma interferon (IFN-γ), which was produced by CD4+ T cells, was observed for the culture supernatant of spleen cells from immune mice, and treatment of immune mice with anti-IFN-γ MAb partially reduced the protection. Moreover, no protection against challenge infection was found in IFN-γ-deficient mice. On the other hand, treatment of immune mice with MAbs against interleukin-2 (IL-2), IL-4, or tumor necrosis factor alpha did not affect protective immunity. These results suggest essential requirements for CD4+ T cells and IFN-γ in protective immunity against challenge infection with B. microti.
机译：小巴贝氏菌在小鼠中产生自限性感染，恢复的小鼠对再感染具有抵抗力。在本研究中，检查了T细胞在针对攻击性感染的保护性免疫中的作用。从原发感染中恢复过来的BALB / c小鼠显示出强大的针对攻击性感染的保护性免疫力。相反，未能控制原发感染并用抗婴儿药治愈的裸鼠未显示出针对攻击性感染的保护作用。用抗CD4单克隆抗体（MAb）治疗免疫小鼠会降低针对攻击性感染的保护性免疫力，但使用抗CD8 MAb的治疗则对保护没有影响。耗竭CD4 + 脾细胞的转移比耗竭CD8 + 脾细胞的转移导致更高的寄生虫病。观察到CD4 + T细胞产生高水平的γ-干扰素（IFN-γ），用于免疫小鼠脾细胞的培养上清液，以及用抗IFN处理免疫小鼠-γMAb部分降低了保护作用。此外，在缺乏IFN-γ的小鼠中未发现针对攻击性感染的保护。另一方面，用抗白介素2（IL-2），IL-4或肿瘤坏死因子α的单克隆抗体治疗免疫小鼠不会影响保护性免疫。这些结果表明，CD4 + T细胞和IFN-γ在抵抗 B激发感染的保护性免疫中必不可少。微量。

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