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首页> 外文期刊>Infection and immunity >Mycoplasmal Lipopeptide MALP-2 Induces the Chemoattractant Proteins Macrophage Inflammatory Protein 1α (MIP-1α), Monocyte Chemoattractant Protein 1, and MIP-2 and Promotes Leukocyte Infiltration in Mice
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Mycoplasmal Lipopeptide MALP-2 Induces the Chemoattractant Proteins Macrophage Inflammatory Protein 1α (MIP-1α), Monocyte Chemoattractant Protein 1, and MIP-2 and Promotes Leukocyte Infiltration in Mice

机译：支原体脂肽MALP-2诱导趋化蛋白巨噬细胞炎性蛋白1α（MIP-1α），单核细胞趋化蛋白1和MIP-2并促进小鼠白细胞浸润

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Natural as well as experimental infections with pathogenic mycoplasmas lead to cellular responses characterized by early polymorphonuclear leukocyte influx, which in turn is followed by infiltration of macrophages. Since some of the most potent leukocyte chemoattractants are macrophage products, we investigated whether the 2-kDa macrophage-activating lipopeptide (MALP-2) from Mycoplasma fermentans was capable of inducing chemoattractant chemokines and initiating an in vivo inflammatory effect. MALP-2 was a potent in vitro inducer of the chemokines macrophage inflammatory protein 1α (MIP-1α), monocyte chemoattractant protein 1 (MCP-1), and MIP-2, yielding a maximal response at 0.1 ng/ml (5 × 10?11 M). Leukocyte infiltration was determined after intraperitoneal injection of MALP-2, liposome-encapsulated MALP-2, and heat-killed mycoplasmas. There was a steady increase in the number of peritoneal cells over 72 h in response to these agents. Polymorph counts were maximal by 24 to 48 h, decreasing thereafter. Monocytes/macrophages had significantly increased after 3 days. MIP-1α, MCP-1, and MIP-2 levels in serum or peritoneal lavage fluid were determined. MIP-1α and MCP-1 levels were elevated by 2 to 6 h after injection and were still above control values after 24 h. In contrast, MIP-2 levels reached their maximum at 2 h, dropping to control values after 24 h. We conclude that macrophage-stimulating mycoplasmal lipoproteins, exemplified by MALP-2, play an important role in the late phase of phagocyte recruitment at sites of infection and that this is affected by leukoattractive chemokines.
机译：天然的和实验性的致病性支原体感染会导致细胞反应，其特征是早期的多形核白细胞大量涌入，继之以巨噬细胞的浸润。由于一些最有效的白细胞趋化因子是巨噬细胞产物，因此我们研究了支原体中的2-kDa活化巨噬细胞脂肽（MALP-2）是否能够诱导趋化因子并启动体内炎症反应影响。 MALP-2是趋化因子巨噬细胞炎症蛋白1α（MIP-1α），单核细胞趋化蛋白1（MCP-1）和MIP-2的有效体外诱导剂，在0.1 ng / ml时产生最大反应（5×10 ？11 M）。腹膜内注射MALP-2，脂质体包裹的MALP-2和热灭活的支原体后，确定白细胞浸润。响应这些药物，在72小时内腹膜细胞的数量稳定增加。多晶型物计数在24至48小时内达到最大值，此后减少。 3天后，单核细胞/巨噬细胞显着增加。测定血清或腹膜灌洗液中的MIP-1α，MCP-1和MIP-2水平。注射后2至6小时MIP-1α和MCP-1水平升高，但24小时后仍高于对照值。相反，MIP-2水平在2 h达到最大值，在24 h后降至控制值。我们得出的结论是，以巨噬细胞刺激的支原体脂蛋白（以MALP-2为例）在感染部位吞噬细胞募集的后期阶段起着重要作用，这受到白细胞吸引趋化因子的影响。

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