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首页> 外文期刊>Infection and immunity >Comparison of Protection in Rabbits against Host-Adapted and Cultivated Borrelia burgdorferi following Infection-Derived Immunity or Immunization with Outer Membrane Vesicles or Outer Surface Protein A
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Comparison of Protection in Rabbits against Host-Adapted and Cultivated Borrelia burgdorferi following Infection-Derived Immunity or Immunization with Outer Membrane Vesicles or Outer Surface Protein A

机译：感染源性免疫或外膜囊泡或外表面蛋白A免疫后，兔对宿主适应和培养的疏螺旋体伯氏疏螺旋体的保护作用的比较

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In this study, infection-derived immunity in the rabbit model of Lyme disease was compared to immunity following immunization with purified outer membrane vesicles (OMV) isolated from Borrelia burgdorferi and recombinant outer surface protein A (OspA). Immunization of rabbits with OMV isolated from virulent strain B31 and its avirulent derivative B313 (lacking OspA and DbpA) conferred highly significant protection against intradermal injection with 6 × 104 in vitro-cultivated virulent B. burgdorferi. This is the first demonstration of protective immunogenicity induced by OMV. While immunization with OspA and avirulent B31 OMV provided far less protection against this challenge, rabbits with infection-derived immunity were completely protected. Protection against host-adapted B. burgdorferi was assessed by implantation of skin biopsies taken from rabbit erythema migrans (a uniquely rich source of B. burgdorferi in vertebrate tissue) containing up to 108 spirochetes. While all of the OMV- and OspA-immunized rabbits were fully susceptible to skin and disseminated infection, rabbits with infection-derived immunity were completely protected. Analysis of the antibody responses to outer membrane proteins, including DbpA, OspA, and OspC, suggests that the remarkable protection exhibited by the infection-immune rabbits is due to antibodies directed at antigens unique to or markedly up-regulated in host-adapted B. burgdorferi.
机译：在这项研究中，将莱姆病兔模型中感染衍生的免疫力与用分离自 Borrelia burgdorferi 的重组外膜囊泡（OMV）和重组外表面蛋白A（OspA）免疫后的免疫力进行了比较。用强毒株B31及其无毒衍生物B313（缺少OspA和DbpA）分离的OMV对兔进行免疫接种，可为6×10 4 体外培养的强毒 B皮内注射提供高度有效的保护。 burgdorferi 。这是OMV诱导的保护性免疫原性的首次证明。虽然用OspA和无毒B31 OMV进行的免疫接种对这种挑战的保护作用要差得多，但是具有感染衍生免疫力的兔子却受到了完全保护。保护免受宿主适应的 B。通过植入兔红斑（皮肤组织中独特的 B。burgdorferi 丰富来源）的皮肤活检样本，对其中的burgdorferi 进行评估，其中最多包含10 8 螺旋体。尽管所有经OMV和OspA免疫的兔子都完全容易受到皮肤和弥散性感染的侵害，但具有感染衍生免疫力的兔子受到了完全保护。对包括DbpA，OspA和OspC在内的外膜蛋白的抗体反应的分析表明，感染免疫兔表现出的显着保护作用是由于针对宿主适应性 B。 burgdorferi 。

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